File:Persistent-cAMP-Signals-Triggered-by-Internalized-G-Protein–Coupled-Receptors-pbio.1000172.s025.ogv
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Persistent-cAMP-Signals-Triggered-by-Internalized-G-Protein–Coupled-Receptors-pbio.1000172.s025.ogv (Ogg Theora video file, length 16 s, 512 × 512 pixels, 34 kbps, file size: 67 KB)
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[edit]DescriptionPersistent-cAMP-Signals-Triggered-by-Internalized-G-Protein–Coupled-Receptors-pbio.1000172.s025.ogv |
English: Effect of dynasore on TSH-Alexa594 internalization. Primary mouse thyroid cells were preincubated with 80 µM dynasore for 20 min and stimulated with 3 µg/ml TSH-Alexa594 for 20 min, in the presence of 80 µM dynasore. After a rapid wash with fresh medium, TSH-Alexa594 fluorescence was visualized with a TIRF microscope set to have a high penetration depth. Data are representative of 25 cells analyzed in three independent experiments. Time between frames was 10 s. Playback is accelerated (one frame/second). |
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Source | Video S9 from Calebiro D, Nikolaev V, Gagliani M, de Filippis T, Dees C, Tacchetti C, Persani L, Lohse M (2009). "Persistent cAMP-Signals Triggered by Internalized G-Protein–Coupled Receptors". PLOS Biology. DOI:10.1371/journal.pbio.1000172. PMID 19688034. PMC: 2718703. | ||
Author | Calebiro D, Nikolaev V, Gagliani M, de Filippis T, Dees C, Tacchetti C, Persani L, Lohse M | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 00:04, 31 October 2012 | 16 s, 512 × 512 (67 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.
Author | Calebiro D, Nikolaev V, Gagliani M, de Filippis T, Dees C, Tacchetti C, Persani L, Lohse M |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2009-08 |