File:Persistent-cAMP-Signals-Triggered-by-Internalized-G-Protein–Coupled-Receptors-pbio.1000172.s023.ogv

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Persistent-cAMP-Signals-Triggered-by-Internalized-G-Protein–Coupled-Receptors-pbio.1000172.s023.ogv ‎(Ogg Theora video file, length 12 s, 512 × 512 pixels, 154 kbps, file size: 226 KB)

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English: Effect of hypertonic sucrose on TSH-Alexa594 internalization. Primary mouse thyroid cells were preincubated with 0.43 M sucrose for 10 min and stimulated with 3 µg/ml TSH-Alexa 594 for 20 min in the presence of 0.43 M sucrose. After a rapid wash with fresh medium, TSH-Alexa594 fluorescence was visualized with a TIRF microscope set to have a high penetration depth. Data are representative of 18 cells analyzed in three independent experiments. Time between frames was 10 s. Playback is accelerated (one frame/second).
Date
Source Video S7 from Calebiro D, Nikolaev V, Gagliani M, de Filippis T, Dees C, Tacchetti C, Persani L, Lohse M (2009). "Persistent cAMP-Signals Triggered by Internalized G-Protein–Coupled Receptors". PLOS Biology. DOI:10.1371/journal.pbio.1000172. PMID 19688034. PMC: 2718703.
Author Calebiro D, Nikolaev V, Gagliani M, de Filippis T, Dees C, Tacchetti C, Persani L, Lohse M
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This file is licensed under the Creative Commons Attribution 2.5 Generic license.
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This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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Date/TimeThumbnailDimensionsUserComment
current00:03, 31 October 201212 s, 512 × 512 (226 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 480P 37 kbps Completed 13:23, 13 October 2018 2.0 s
Streaming 480p (VP9) Not ready Unknown status
VP9 360P 16 kbps Completed 13:23, 13 October 2018 1.0 s
Streaming 360p (VP9) Not ready Unknown status
VP9 240P 8 kbps Completed 13:23, 13 October 2018 1.0 s
Streaming 240p (VP9) 8 kbps Completed 12:22, 5 December 2023 1.0 s
WebM 360P 174 kbps Completed 12:07, 19 November 2012 1.0 s
Streaming 144p (MJPEG) 28 kbps Completed 17:16, 18 November 2023 1.0 s

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