File:Permanent-Occlusion-of-Feeding-Arteries-and-Draining-Veins-in-Solid-Mouse-Tumors-by-Vascular-pone.0010282.s001.ogv

English: Dynamic light scattering imaging of the mouse earlobe during Tookad-VTP. In this video clip, the images of the mouse ear blood vessel were produced after temporal contrast calculations, as previously described (Kalchenko V, Preise D, Bayewitch M, Fine I, Burd K, Harmelin A. In vivo dynamic light scattering microscopy of tumour blood vessels. J Microsc. 2007;228:118–122) and presented as a color coded map (higher perfusion is illustrated by lighter colors). Normal perfusion is apparent throughout the untreated control (−120 sec to −60 sec) and light control (−60 sec to 0 sec) periods. Tookad was injected at time zero. With progression of Tookad-VTP, a selective and gradual reduction in tumor perfusion was observed (60 sec). Towards the end of the treatment session (200 sec), absence of perfusion was detected in the tumor zone alone, while the surrounding vasculature remained functional throughout. All other details are described in the Materials and Methods section.