File:Pathways-of-allosteric-regulation-in-Hsp70-chaperones-ncomms9308-s4.ogv

English: Supplementary Movie 3 Supplementary Movie 3 (related to Fig. 5): ATP induced dynamics of the NBD. The movie shows a morph starting from an homology model of E. coli DnaK onto the structure of bovine Hsc70 in complex with ADP and phosphate (PDB ID 1HPM) to the ATP bound open conformation of DnaK (PDB ID 4B9Q) and back. Surface representation with lobe I in dark gray, lobe II in light gray, and residues interacting with SBDβ colored according effects on basal ATPase rate upon replacement with alanine (Y145A, F146A, D148A, R151A, K155A, R167A) or upon replacement of interacting residues (I168[D481A], D326[K414I]). Increase in basal ATPase rate less than 5-fold (yellow). 5 to 10-fold (orange), 11 to 15-fold (red), 16 to 20-fold (dark red), more than 26 to 84-fold (magenta). Morph was created on The Yale Morph Server (http://molmovdb.org/morph/) and visualized in PyMOL (The PyMOL Molecular Graphics System, Version 1.7.4 Schrödinger, LLC).