File:Parathyroid-Hormone-Induces-Bone-Cell-Motility-and-Loss-of-Mature-Osteocyte-Phenotype-through-L-pone.0125731.s005.ogv
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Size of this JPG preview of this OGG file: 800 × 299 pixels. Other resolutions: 320 × 120 pixels | 640 × 239 pixels | 1,392 × 520 pixels.
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[edit]DescriptionParathyroid-Hormone-Induces-Bone-Cell-Motility-and-Loss-of-Mature-Osteocyte-Phenotype-through-L-pone.0125731.s005.ogv |
English: PTH-induces an elongated shape and increased motility in mature IDG-SW3 cells. IDG-SW3 cells were differentiated for 28 days and the mineral was imaged by adding 0.5μg/ml alizarin red to the culture media as a vital stain for calcium. The left panel shows control cultures treated with the PBS vehicle and the right panel shows cultures treated with 50nM PTH 1–34. Time lapse images were captured using a widefield epifluorescence live imaging microscope every 30 minutes. Note the dramatic elongation of the Dmp1-GFP positive cells in the PTH treated culture and the increased motility of these elongated cells compared to the control. |
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Source | S1 Video from Prideaux M, Dallas S, Zhao N, Johnsrud E, Veno P, Guo D, Mishina Y, Harris S, Bonewald L (2015). "Parathyroid Hormone Induces Bone Cell Motility and Loss of Mature Osteocyte Phenotype through L-Calcium Channel Dependent and Independent Mechanisms". PLOS ONE. DOI:10.1371/journal.pone.0125731. PMID 25942444. PMC: 4420268. | ||
Author | Prideaux M, Dallas S, Zhao N, Johnsrud E, Veno P, Guo D, Mishina Y, Harris S, Bonewald L | ||
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![]() ![]() This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 23:22, 19 May 2015 | 18 s, 1,392 × 520 (2.83 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | PTH-induces an elongated shape and increased motility in mature IDG-SW3 cells. |
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Author | Prideaux M, Dallas S, Zhao N, Johnsrud E, Veno P, Guo D, Mishina Y, Harris S, Bonewald L |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | IDG-SW3 cells were differentiated for 28 days and the mineral was imaged by adding 0.5μg/ml alizarin red to the culture media as a vital stain for calcium. The left panel shows control cultures treated with the PBS vehicle and the right panel shows cultures treated with 50nM PTH 1–34. Time lapse images were captured using a widefield epifluorescence live imaging microscope every 30 minutes. Note the dramatic elongation of the Dmp1-GFP positive cells in the PTH treated culture and the increased motility of these elongated cells compared to the control. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2015-05-05 |