File:Parathyroid-Hormone-Induces-Bone-Cell-Motility-and-Loss-of-Mature-Osteocyte-Phenotype-through-L-pone.0125731.s005.ogv

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Original file ‎(Ogg Theora video file, length 18 s, 1,392 × 520 pixels, 1.3 Mbps, file size: 2.83 MB)

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English: PTH-induces an elongated shape and increased motility in mature IDG-SW3 cells. IDG-SW3 cells were differentiated for 28 days and the mineral was imaged by adding 0.5μg/ml alizarin red to the culture media as a vital stain for calcium. The left panel shows control cultures treated with the PBS vehicle and the right panel shows cultures treated with 50nM PTH 1–34. Time lapse images were captured using a widefield epifluorescence live imaging microscope every 30 minutes. Note the dramatic elongation of the Dmp1-GFP positive cells in the PTH treated culture and the increased motility of these elongated cells compared to the control.
Date
Source S1 Video from Prideaux M, Dallas S, Zhao N, Johnsrud E, Veno P, Guo D, Mishina Y, Harris S, Bonewald L (2015). "Parathyroid Hormone Induces Bone Cell Motility and Loss of Mature Osteocyte Phenotype through L-Calcium Channel Dependent and Independent Mechanisms". PLOS ONE. DOI:10.1371/journal.pone.0125731. PMID 25942444. PMC: 4420268.
Author Prideaux M, Dallas S, Zhao N, Johnsrud E, Veno P, Guo D, Mishina Y, Harris S, Bonewald L
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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Date/TimeThumbnailDimensionsUserComment
current23:22, 19 May 201518 s, 1,392 × 520 (2.83 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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VP9 720P 519 kbps Completed 15:40, 6 September 2018 12 s
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VP9 480P 261 kbps Completed 15:39, 6 September 2018 9.0 s
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VP9 360P 138 kbps Completed 15:39, 6 September 2018 6.0 s
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VP9 240P 80 kbps Completed 15:39, 6 September 2018 6.0 s
Streaming 240p (VP9) 81 kbps Completed 05:58, 5 December 2023 1.0 s
WebM 360P 519 kbps Completed 09:14, 1 December 2016 12 s
Streaming 144p (MJPEG) 597 kbps Completed 05:17, 18 November 2023 1.0 s

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