File:Organizational-Changes-of-the-Daughter-Basal-Complex-during-the-Parasite-Replication-of-Toxoplasma-ppat.0040010.sv002.ogv

English: The Initial Construction of the TgMORN1 Ring Is Likely to Be Independent of the Structural Integrity of the Daughter Cortical Cytoskeleton A parasitophorous vacuole containing eight dividing parasites expressing EGFP-TgMORN1 (green) and mCherryFP-TgTubA1 (red) treated with 2.5 μM oryzalin. This concentration of oryzalin was shown previously to inhibit the formation of the spindle and the daughter cortical microtubules, but not the centriole replication, during T. gondii cell division [9]. No daughter cortical microtubules can be detected in these parasites. The initiation (33–48 min) and construction (60–120 min) of the basal complex are not affected. Notice that the mother's conoid, cortical microtubules, and basal complex are not affected by the oryzalin treatment, and the overall shape of the mother cell remains normal (0–135 min) until the distorted daughters attempt to bud (150–165 min). As previously reported [9,17], the spindle pole fails to replicate with 2.5 μM oryzalin treatment. Images were collected at 37 °C. All images except for the first two time-points were collected at ∼15 min intervals. The time interval between the first two time-points was 33 min. The total elapsed time for the video sequence is 4 h and 45 min. All images in the video are maximum intensity projections of deconvolved 3D stacks.