File:Organizational-Changes-of-the-Daughter-Basal-Complex-during-the-Parasite-Replication-of-Toxoplasma-ppat.0040010.sv001.ogv
Organizational-Changes-of-the-Daughter-Basal-Complex-during-the-Parasite-Replication-of-Toxoplasma-ppat.0040010.sv001.ogv (Ogg Theora video file, length 9.0 s, 544 × 206 pixels, 120 kbps, file size: 132 KB)
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[edit]DescriptionOrganizational-Changes-of-the-Daughter-Basal-Complex-during-the-Parasite-Replication-of-Toxoplasma-ppat.0040010.sv001.ogv |
English: Time-lapse Images Showing That the TgMORN1 Ring Is Initiated around the Duplicated Centrioles This video shows a time-lapse experiment tracking the cell division of four parasites expressing EGFP-TgMORN1 (green) and mCherryFP-TgTubA1 (red). At time 0, centriole duplication (red arrows) has occurred, and in addition to TgMORN1 fluorescence in the spindle poles, there are two small extra masses of EGFP-TgMORN1 fluorescence (green arrows), one located on each side of the spindle pole, overlapping with the centriole labeling by mCherryFP-TgTubA1 (red arrows). Twenty to 30 min later, the fluorescence of mCherryFP-TgTubA1 in the centriole increases (red arrows), likely correlated with the initial assembly of the conoid in the apical complex, of which TgTubA1 is a major component. At this point, the ring-like nature of the TgMORN1 containing structure becomes apparent, and it is centered around the centriole/conoid mass (green arrows, t = 40 min). The TgMORN1 rings then undergo further expansion. At t = 60 min, the centriole/conoid assemblies move apically above the plane of the TgMORN1 ring with the extension of the cortical microtubules, and the recruitment of TgMORN1 to the apical complex becomes clear (green arrowheads). TgMORN1 rings clearly start to constrict (t = 2 h 32 min) before the onset of cytokinesis (t = 2 h 42 min). Insets: 2× magnification of regions indicated by the arrows. The images were collected at ∼10 min intervals at 37 °C. The total elapsed time for the video sequence is 2 h and 52 min. All images in the video are maximum intensity projections of deconvolved 3D stacks. |
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Source | Video S1 from Hu K (2008). "Organizational Changes of the Daughter Basal Complex during the Parasite Replication of Toxoplasma gondii". PLOS Pathogens. DOI:10.1371/journal.ppat.0040010. PMID 18208326. PMC: 2211554. | ||
Author | Hu K | ||
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current | 00:59, 14 November 2012 | 9.0 s, 544 × 206 (132 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Time-lapse Images Showing That the TgMORN1 Ring Is Initiated around the Duplicated Centrioles |
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Author | Hu K |
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | This video shows a time-lapse experiment tracking the cell division of four parasites expressing EGFP-TgMORN1 (green) and mCherryFP-TgTubA1 (red). At time 0, centriole duplication (red arrows) has occurred, and in addition to TgMORN1 fluorescence in the spindle poles, there are two small extra masses of EGFP-TgMORN1 fluorescence (green arrows), one located on each side of the spindle pole, overlapping with the centriole labeling by mCherryFP-TgTubA1 (red arrows). Twenty to 30 min later, the fluorescence of mCherryFP-TgTubA1 in the centriole increases (red arrows), likely correlated with the initial assembly of the conoid in the apical complex, of which TgTubA1 is a major component. At this point, the ring-like nature of the TgMORN1 containing structure becomes apparent, and it is centered around the centriole/conoid mass (green arrows, t |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2008-01 |