File:One-Small-Step-for-a-Yeast---Microevolution-within-Macrophages-Renders-Candida-glabrata-ppat.1004478.s014.ogv
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One-Small-Step-for-a-Yeast---Microevolution-within-Macrophages-Renders-Candida-glabrata-ppat.1004478.s014.ogv (Ogg Theora video file, length 1 min 26 s, 640 × 480 pixels, 2.3 Mbps, file size: 23.56 MB)
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[edit]DescriptionOne-Small-Step-for-a-Yeast---Microevolution-within-Macrophages-Renders-Candida-glabrata-ppat.1004478.s014.ogv |
English: Wt yeasts are mostly cleared by macrophages and surviving cells escape the phagocytes later than the Evo strain. Macrophages were infected with sonicated wild type yeast cells at a MOI of 2∶1. After 60 min of phagocytosis, non-attached yeasts were washed away and time-lapse microscopy was started. The majority of yeasts were cleared by macrophages within 48 hours (green arrows; 1, clearance after 7 h; 2, clearance after 40 h). Only some yeasts survived and replicated intracellularly (red arrow). After approximately 4 d, the number of extracellular yeasts increased and macrophages finally burst (examples shown with black arrows in temporal order). |
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Source | Video S2 from Brunke S, Seider K, Fischer D, Jacobsen I, Kasper L, Jablonowski N, Wartenberg A, Bader O, Enache-Angoulvant A, Schaller M, d'Enfert C, Hube B (2014). "One Small Step for a Yeast - Microevolution within Macrophages Renders Candida glabrata Hypervirulent Due to a Single Point Mutation". PLOS Pathogens. DOI:10.1371/journal.ppat.1004478. PMID 25356907. PMC: 4214790. | ||
Author | Brunke S, Seider K, Fischer D, Jacobsen I, Kasper L, Jablonowski N, Wartenberg A, Bader O, Enache-Angoulvant A, Schaller M, d'Enfert C, Hube B | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 02:46, 14 November 2014 | 1 min 26 s, 640 × 480 (23.56 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Brunke S, Seider K, Fischer D, Jacobsen I, Kasper L, Jablonowski N, Wartenberg A, Bader O, Enache-Angoulvant A, Schaller M, d'Enfert C, Hube B |
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Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Wt yeasts are mostly cleared by macrophages and surviving cells escape the phagocytes later than the Evo strain. Macrophages were infected with sonicated wild type yeast cells at a MOI of 2∶1. After 60 min of phagocytosis, non-attached yeasts were washed away and time-lapse microscopy was started. The majority of yeasts were cleared by macrophages within 48 hours (green arrows; 1, clearance after 7 h; 2, clearance after 40 h). Only some yeasts survived and replicated intracellularly (red arrow). After approximately 4 d, the number of extracellular yeasts increased and macrophages finally burst (examples shown with black arrows in temporal order). |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2014-10 |