File:One-Small-Step-for-a-Yeast---Microevolution-within-Macrophages-Renders-Candida-glabrata-ppat.1004478.s013.ogv

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One-Small-Step-for-a-Yeast---Microevolution-within-Macrophages-Renders-Candida-glabrata-ppat.1004478.s013.ogv ‎(Ogg Theora video file, length 59 s, 640 × 480 pixels, 2.19 Mbps, file size: 15.32 MB)

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English: The Evo strain escapes RAW 246.7 macrophages by pseudohyphal outgrowth. Macrophages were infected with sonicated cells of the Evo strain at a MOI of 2∶1. After 60 min of phagocytosis, non-attached yeasts were washed away and time-lapse microscopy was started. Phagocytosed fungal cells continued to grow in a pseudohyphae-like morphology within the macrophages (red arrow), which finally led to host cell rupture and the attraction of numerous macrophages (white arrows). Starting at approximately 48 h, macrophages burst (examples shown by black arrows 1–4) and C. glabrata cells escaped to overgrow the macrophages.
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Source Video S1 from Brunke S, Seider K, Fischer D, Jacobsen I, Kasper L, Jablonowski N, Wartenberg A, Bader O, Enache-Angoulvant A, Schaller M, d'Enfert C, Hube B (2014). "One Small Step for a Yeast - Microevolution within Macrophages Renders Candida glabrata Hypervirulent Due to a Single Point Mutation". PLOS Pathogens. DOI:10.1371/journal.ppat.1004478. PMID 25356907. PMC: 4214790.
Author Brunke S, Seider K, Fischer D, Jacobsen I, Kasper L, Jablonowski N, Wartenberg A, Bader O, Enache-Angoulvant A, Schaller M, d'Enfert C, Hube B
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Date/TimeThumbnailDimensionsUserComment
current02:44, 14 November 201459 s, 640 × 480 (15.32 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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VP9 480P 1.12 Mbps Completed 21:58, 5 September 2018 45 s
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VP9 240P 301 kbps Completed 21:58, 5 September 2018 35 s
Streaming 240p (VP9) 301 kbps Completed 18:10, 6 February 2024 2.0 s
WebM 360P 505 kbps Completed 02:45, 14 November 2014 42 s
Streaming 144p (MJPEG) 710 kbps Completed 06:20, 17 November 2023 2.0 s

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