File:Nucleocapsid-Promotes-Localization-of-HIV-1-Gag-to-Uropods-That-Participate-in-Virological-Synapses-ppat.1001167.s009.ogv

English: Gag puncta move to and accumulate at a forming uropod in the absence of a crosslinking antibody. Time lapse images of a Gag-YFP-expressing T cell during repolarization. Gag-YFP(green)-expressing P2 cells were depolarized by incubation at 4°C for 30 min. Approximately 5 min after chamber coverslips containing depolarized cells were transferred to the microscope stage maintained at 37°C, acquisition of live cell images at 30-s intervals was begun and continued for 26 min. Note that even as the cell rotates and changes direction, the small patches migrate and coalesce to the large patch at the cell pole that eventually forms the uropod.