File:Nucleocapsid-Promotes-Localization-of-HIV-1-Gag-to-Uropods-That-Participate-in-Virological-Synapses-ppat.1001167.s008.ogv

English: Cell surface patches containing Gag and a uropod marker laterally move to and accumulate at a forming uropod. Time lapse images of a Gag-YFP-expressing T cell during repolarization. Gag-YFP(green)-expressing P2 cells were immunostained for PSGL-1 (red) as described in Figure 6. Cells were then depolarized by incubation at 4°C for 30 min. Approximately 5 min after chamber coverslips containing depolarized cells were transferred to the microscope stage maintained at 37°C, acquisition of live cell images at 30-s intervals was begun and continued for 27 min. Note that the small patches migrate and coalesce to the large patch at the cell pole that eventually forms the uropod.