File:Novel-Roles-of-Formin-mDia2-in-Lamellipodia-and-Filopodia-Formation-in-Motile-Cells-pbio.0050317.sv004.ogv
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Novel-Roles-of-Formin-mDia2-in-Lamellipodia-and-Filopodia-Formation-in-Motile-Cells-pbio.0050317.sv004.ogv (Ogg Theora video file, length 6.1 s, 227 × 328 pixels, 510 kbps, file size: 380 KB)
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[edit]DescriptionNovel-Roles-of-Formin-mDia2-in-Lamellipodia-and-Filopodia-Formation-in-Motile-Cells-pbio.0050317.sv004.ogv |
English: Kinetics of GFP-Actin during Filopodia Formation Induced by mRFP-ΔGBD-mDia2 in B16F1 Cell Coexpression of GFP-actin (white) and mRFP1-ΔGBD-mDia2 (not shown) in B16F1 cell shows actin enrichment at the thicker termini of GFP-ΔGBD-mDia2–induced filopodia. Arrow points to an actin speckle which undergoes slow retrograde flow and finally disappears, while the filopodial tip protrudes forward. This behavior illustrates actin assembly at the tip, whereas disappearance of the speckles and overall decrease of actin intensity toward the rear indicates actin disassembly in proximal regions. This video corresponds to Figure 6F–6H. |
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Source | Video S4 from Yang C, Czech L, Gerboth S, Kojima S, Scita G, Svitkina T (2007). "Novel Roles of Formin mDia2 in Lamellipodia and Filopodia Formation in Motile Cells". PLOS Biology. DOI:10.1371/journal.pbio.0050317. PMID 18044991. PMC: 2229861. | ||
Author | Yang C, Czech L, Gerboth S, Kojima S, Scita G, Svitkina T | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 23:15, 30 October 2012 | 6.1 s, 227 × 328 (380 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Kinetics of GFP-Actin during Filopodia Formation Induced by mRFP-?GBD-mDia2 in B16F1 Cell |
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Author | Yang C, Czech L, Gerboth S, Kojima S, Scita G, Svitkina T |
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Coexpression of GFP-actin (white) and mRFP1-?GBD-mDia2 (not shown) in B16F1 cell shows actin enrichment at the thicker termini of GFP-?GBD-mDia2?induced filopodia. Arrow points to an actin speckle which undergoes slow retrograde flow and finally disappears, while the filopodial tip protrudes forward. This behavior illustrates actin assembly at the tip, whereas disappearance of the speckles and overall decrease of actin intensity toward the rear indicates actin disassembly in proximal regions. This video corresponds to Figure 6F?6H. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2007-11 |