File:Nematode C. elegans Extra chromosomal array expression and histone modifications in the germline.jpg
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DescriptionNematode C. elegans Extra chromosomal array expression and histone modifications in the germline.jpg | Figure 4. Extrachromosomal array expression and histone modifications in the germline. Repetitive extrachromosomal transgenes are often silenced in adult germ cell nuclei. Silencing of transgene arrays can be alleviated by decreasing the copy number of the reporter transgene, and increasing the “complexity” of the array DNA. Panels A and B show a DIC image (A) and GFP fluorescence (B) of a transgenic animal with a “complex” array carrying a let-858::gfp reporter-a gene that should express in all cells. Panel C shows the expression pattern of the same reporter in a repetitive transgene. The germ cell nuclei in the gonad are circled in A-C. The silenced, repetitive transgene in the strain depicted in C is depleted of H3methK4 (D) and enriched in H3methK9 (F); arrows in D-G point to the transgene. Panels D and G: Red is DAPI; Green is antibody signal for the respective histone modifications. Panels E and F are DAPI stains of Panels D and G, respectively. |
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Source | http://www.wormbook.org/chapters/www_germlinechromatin/germlinechromatin.html Schaner, C. E. and Kelly, W. G. Germline chromatin (January 24, 2006), WormBook, ed. The C. elegans Research Community, WormBook, doi/10.1895/wormbook.1.73.1, http://www.wormbook.org. |
Author | Schaner, C. E. and Kelly, W. G. |
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current | 15:59, 27 February 2024 | ![]() | 1,800 × 678 (575 KB) | Rasbak (talk | contribs) | {{Information |description={{Information |description=Figure 4. Extrachromosomal array expression and histone modifications in the germline. Repetitive extrachromosomal transgenes are often silenced in adult germ cell nuclei. Silencing of transgene arrays can be alleviated by decreasing the copy number of the reporter transgene, and increasing the “complexity” of the array DNA. Panels A and B show a DIC image (A) and GFP fluorescence (B) of a transgenic animal with a “complex” array carrying... |
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Software used | Adobe Photoshop 7.0 |
File change date and time | 10:27, 13 January 2006 |
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IIM version | 2 |