File:NKp46-Clusters-at-the-Immune-Synapse-and-Regulates-NK-Cell-Polarization-Video 1.ogv
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[edit]DescriptionNKp46-Clusters-at-the-Immune-Synapse-and-Regulates-NK-Cell-Polarization-Video 1.ogv |
English: Lytic granules tracking in live cell imaging microscopy. Target cells were pre-labeled with DiD membrane dye (red) and co-incubated with NK92.p46-IRES-GFP (green) pre-loaded with LysoTracker DND99 (gray) on a δT dish at 37°C. Time lapse images of random fields were recorded over 30–90 min at frame per 10 s rate. Granules polarization time was determined by minimal distance to the immune synapse as previously described (Mentlik MBOC 2010). Representative composite video shows time from initial interaction at 7 frames/s rate. |
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Source | Video S1 from Hadad U, Thauland T, Martinez O, Butte M, Porgador A, Krams S (2015). "NKp46 Clusters at the Immune Synapse and Regulates NK Cell Polarization". Frontiers in Immunology. DOI:10.3389/fimmu.2015.00495. PMID 26441997. PMC: 4585260. | ||
Author | Hadad U, Thauland T, Martinez O, Butte M, Porgador A, Krams S | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 01:22, 12 October 2015 | 15 s, 854 × 480 (952 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Hadad U, Thauland T, Martinez O, Butte M, Porgador A, Krams S |
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Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Lytic granules tracking in live cell imaging microscopy. Target cells were pre-labeled with DiD membrane dye (red) and co-incubated with NK92.p46-IRES-GFP (green) pre-loaded with LysoTracker DND99 (gray) on a δT dish at 37°C. Time lapse images of random fields were recorded over 30–90 min at frame per 10 s rate. Granules polarization time was determined by minimal distance to the immune synapse as previously described (Mentlik MBOC 2010). Representative composite video shows time from initial interaction at 7 frames/s rate. |
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Date and time of digitizing | 2015-09-25 |