File:Multiplex-Profiling-of-Cellular-Invasion-in-3D-Cell-Culture-Models-pone.0063121.s008.ogv
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English: 4D invasion assay. Confocal 4D (z-stacks over time) time-lapse movie that was rendered as a maximum intensity projection with Imaris software. Each large graphical object depicted in red represents one cell whereas single cells are observed to invade 50–100 µm deep into the 3D collagen gel (see also Fig. 2 and Fig. S2). Small speckle-like objects, that are visible throughout the whole movie, represent background noise due to image acquisition. MLg lung fibroblasts were stained with the cell tracker dye CMTPX. Recording started within one hour after seeding the cells on top of the 3D collagen matrix and z-stacks were acquired in 30 minutes intervals. Scale bar, 70 µm. |
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| Source | Movie S3 from Burgstaller G, Oehrle B, Koch I, Lindner M, Eickelberg O (2013). "Multiplex Profiling of Cellular Invasion in 3D Cell Culture Models". PLOS ONE. DOI:10.1371/journal.pone.0063121. PMID 23671660. PMC: 3650046. | ||
| Author | Burgstaller G, Oehrle B, Koch I, Lindner M, Eickelberg O | ||
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 04:31, 18 May 2013 | 18 s, 1,280 × 720 (2.17 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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