File:Multicellular-Bacteria-Deploy-the-Type-VI-Secretion-System-to-Preemptively-Strike-Neighboring-Cells-ppat.1003608.s010.ogv
Multicellular-Bacteria-Deploy-the-Type-VI-Secretion-System-to-Preemptively-Strike-Neighboring-Cells-ppat.1003608.s010.ogv (Ogg Theora video file, length 32 s, 480 × 360 pixels, 527 kbps, file size: 2.01 MB)
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[edit]DescriptionMulticellular-Bacteria-Deploy-the-Type-VI-Secretion-System-to-Preemptively-Strike-Neighboring-Cells-ppat.1003608.s010.ogv |
English: Merger of opposing swarming populations of P. mirabilis. Live-cell fluorescence microscopy captures the merging of multicellular swarming populations of P. mirabilis wild-type HI4230 and mutant 9C1. P. mirabilis HI4230 constitutively expressing green fluorescent protein (GFP) and cultures of mutant 9C1 constitutively expressing dsRed were spotted on opposite sides of a swarm agar plate and were observed in real-time under brightfield and fluorescence microscopy as the bacterial strains swarmed toward one another. The strains clearly merge and seamlessly interdigitate as cells from opposing swarms first come into direct cell-cell contact immediately upon merging. Single channel fluorescence, brightfield, and merged images are presented. |
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Source | Movie S1 from Alteri C, Himpsl S, Pickens S, Lindner J, Zora J, Miller J, Arno P, Straight S, Mobley H (2013). "Multicellular Bacteria Deploy the Type VI Secretion System to Preemptively Strike Neighboring Cells". PLOS Pathogens. DOI:10.1371/journal.ppat.1003608. PMC: 3764213. | ||
Author | Alteri C, Himpsl S, Pickens S, Lindner J, Zora J, Miller J, Arno P, Straight S, Mobley H | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 20:29, 14 September 2013 | 32 s, 480 × 360 (2.01 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Alteri C, Himpsl S, Pickens S, Lindner J, Zora J, Miller J, Arno P, Straight S, Mobley H |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Merger of opposing swarming populations of P. mirabilis. Live-cell fluorescence microscopy captures the merging of multicellular swarming populations of P. mirabilis wild-type HI4230 and mutant 9C1. P. mirabilis HI4230 constitutively expressing green fluorescent protein (GFP) and cultures of mutant 9C1 constitutively expressing dsRed were spotted on opposite sides of a swarm agar plate and were observed in real-time under brightfield and fluorescence microscopy as the bacterial strains swarmed toward one another. The strains clearly merge and seamlessly interdigitate as cells from opposing swarms first come into direct cell-cell contact immediately upon merging. Single channel fluorescence, brightfield, and merged images are presented. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013-09 |