File:Monoclonal-Antibodies-13A4-and-AC133-Do-Not-Recognize-the-Canine-Ortholog-of-Mouse-and-Human-Stem-pone.0164079.s008.ogv
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Monoclonal-Antibodies-13A4-and-AC133-Do-Not-Recognize-the-Canine-Ortholog-of-Mouse-and-Human-Stem-pone.0164079.s008.ogv (Ogg Theora video file, length 18 s, 640 × 480 pixels, 138 kbps, file size: 310 KB)
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[edit]DescriptionMonoclonal-Antibodies-13A4-and-AC133-Do-Not-Recognize-the-Canine-Ortholog-of-Mouse-and-Human-Stem-pone.0164079.s008.ogv |
English: Localization of canine prominin-1-GFP in microvilli and the primary cilium of MDCK cells. Confluent MDCK cells expressing canine prominin-1-GFP (green) were fixed, permeabilized and immunolabeled with mAb against acetylated α-tubulin (red) to reveal the primary cilium. Their nuclei were counterstained with DAPI (blue). Image was acquired by confocal laser scanning microscope and processed using Volocity software. |
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Source | S1 Video from Thamm K, Graupner S, Werner C, Huttner W, Corbeil D (2016). "Monoclonal Antibodies 13A4 and AC133 Do Not Recognize the Canine Ortholog of Mouse and Human Stem Cell Antigen Prominin-1 (CD133)". PLOS ONE. DOI:10.1371/journal.pone.0164079. PMID 27701459. PMC: 5049760. | ||
Author | Thamm K, Graupner S, Werner C, Huttner W, Corbeil D | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 04:23, 29 October 2016 | 18 s, 640 × 480 (310 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Localization of canine prominin-1-GFP in microvilli and the primary cilium of MDCK cells. |
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Author | Thamm K, Graupner S, Werner C, Huttner W, Corbeil D |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Confluent MDCK cells expressing canine prominin-1-GFP (green) were fixed, permeabilized and immunolabeled with mAb against acetylated α-tubulin (red) to reveal the primary cilium. Their nuclei were counterstained with DAPI (blue). Image was acquired by confocal laser scanning microscope and processed using Volocity software. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2016-10-04 |
Categories:
- Spectrum analysis techniques
- Flow cytometry
- Immunohistochemistry
- Immunologic techniques
- Videos of Mardin-Darby canine kidney cells
- Videos of membrane proteins
- Forms of DNA
- Complementary DNA
- Videos of nucleic acids
- Videos of molecular biology
- Videos of molecular biology techniques
- Molecular probe techniques
- Videos of urinary system
- Media from PLOS ONE