File:Monitoring-the-Systemic-Human-Memory-B-Cell-Compartment-of-Melanoma-Patients-for-Anti-Tumor-IgG-pone.0019330.s004.ogv
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[edit]DescriptionMonitoring-the-Systemic-Human-Memory-B-Cell-Compartment-of-Melanoma-Patients-for-Anti-Tumor-IgG-pone.0019330.s004.ogv |
English: Real-time live-cell cytotoxicity assay for the 6_2D10 non-melanoma-specific
antibody (negative control). (AVI) Videos S1 and S2 These video files show our real-time cytotoxic assays. Video S1 shows real-time functional data of the 6_2G3 melanoma-specific patient derived antibody which was observed to kill melanoma cells. Video S2 shows the identical assays shown in Video S1 using a non-melanoma specific antibody derived from the same patient, as a negative control. In these assays, live tumor cells are labeled in green (live cell dye), U-937 monocytic cells are labeled in blue, and cell death is indicated by the incorporation of red (Ethidium homodimer-1 incorporation). Frames from these videos are also displayed in Figure 7, B. |
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Source | Video S2 from Gilbert A, Karagiannis P, Dodev T, Koers A, Lacy K, Josephs D, Takhar P, Geh J, Healy C, Harries M, Acland K, Rudman S, Beavil R, Blower P, Beavil A, Gould H, Spicer J, Nestle F, Karagiannis S (2011). "Monitoring the Systemic Human Memory B Cell Compartment of Melanoma Patients for Anti-Tumor IgG Antibodies". PLOS ONE. DOI:10.1371/journal.pone.0019330. PMID 21559411. PMC: 3084832. | ||
Author | Gilbert A, Karagiannis P, Dodev T, Koers A, Lacy K, Josephs D, Takhar P, Geh J, Healy C, Harries M, Acland K, Rudman S, Beavil R, Blower P, Beavil A, Gould H, Spicer J, Nestle F, Karagiannis S | ||
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current | 02:51, 29 May 2013 | 4.0 s, 1,388 × 1,040 (925 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Gilbert A, Karagiannis P, Dodev T, Koers A, Lacy K, Josephs D, Takhar P, Geh J, Healy C, Harries M, Acland K, Rudman S, Beavil R, Blower P, Beavil A, Gould H, Spicer J, Nestle F, Karagiannis S |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Real-time live-cell cytotoxicity assay for the 6_2D10 non-melanoma-specific
antibody (negative control). (AVI) Videos S1 and S2 These video files show our real-time cytotoxic assays. Video S1 shows real-time functional data of the 6_2G3 melanoma-specific patient derived antibody which was observed to kill melanoma cells. Video S2 shows the identical assays shown in Video S1 using a non-melanoma specific antibody derived from the same patient, as a negative control. In these assays, live tumor cells are labeled in green (live cell dye), U-937 monocytic cells are labeled in blue, and cell death is indicated by the incorporation of red (Ethidium homodimer-1 incorporation). Frames from these videos are also displayed in Figure 7, B. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2011 |
- Videos of leukocytes
- Plasma cells
- Videos of B cells
- Immune physiology
- Clinical immunology
- Immune response
- Videos of melanoma
- Videos of cancer treatments
- Antibody therapy
- Monoclonal antibodies
- Case-control studies
- Tumor cell line
- Cohort studies
- Disease progression
- Enzyme-linked immunosorbent assay
- Videos of immune system
- Immunoglobulin G
- Time factors
- Media from PLOS ONE
- Enzyme assays