File:Monitoring-the-Systemic-Human-Memory-B-Cell-Compartment-of-Melanoma-Patients-for-Anti-Tumor-IgG-pone.0019330.s004.ogv

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English: Real-time live-cell cytotoxicity assay for the 6_2D10 non-melanoma-specific

antibody (negative control). (AVI) Videos S1 and S2 These video files show our real-time cytotoxic assays. Video S1 shows real-time functional data of the 6_2G3 melanoma-specific patient derived antibody which was observed to kill melanoma cells. Video S2 shows the identical assays shown in Video S1 using a non-melanoma specific antibody derived from the same patient, as a negative control. In these assays, live tumor cells are labeled in green (live cell dye), U-937 monocytic cells are labeled in blue, and cell death is indicated by the incorporation of red (Ethidium homodimer-1 incorporation). Frames from these videos are also displayed in Figure 7,

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Source Video S2 from Gilbert A, Karagiannis P, Dodev T, Koers A, Lacy K, Josephs D, Takhar P, Geh J, Healy C, Harries M, Acland K, Rudman S, Beavil R, Blower P, Beavil A, Gould H, Spicer J, Nestle F, Karagiannis S (2011). "Monitoring the Systemic Human Memory B Cell Compartment of Melanoma Patients for Anti-Tumor IgG Antibodies". PLOS ONE. DOI:10.1371/journal.pone.0019330. PMID 21559411. PMC: 3084832.
Author Gilbert A, Karagiannis P, Dodev T, Koers A, Lacy K, Josephs D, Takhar P, Geh J, Healy C, Harries M, Acland K, Rudman S, Beavil R, Blower P, Beavil A, Gould H, Spicer J, Nestle F, Karagiannis S
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This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.
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VP9 720P 350 kbps Completed 17:50, 3 September 2018 4.0 s
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