File:Monitoring-the-Cytoskeletal-EGF-Response-in-Live-Gastric-Carcinoma-Cells-pone.0045280.s001.ogv
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[edit]DescriptionMonitoring-the-Cytoskeletal-EGF-Response-in-Live-Gastric-Carcinoma-Cells-pone.0045280.s001.ogv |
English: Ruffling activity is increased upon addition of EGF. MKN1 and Hs746T cells were transfected to produce enhanced yellow fluorescence protein (YFP) under CMV-promoter control. The fluorescence was recorded by confocal laser scanning microscopy three times per minute in the absence of EGF and after addition of 5 ng/ml EGF (corresponding Fig. 1A–B’). Note the EGF-induced cell spreading and the appearance of multiple ruffles and lamellipodia that leads to extensive lamella formation in MKN1 cells but not in Hs746T cells. |
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Source | Video S1 from Felkl M, Tomas K, Smid M, Mattes J, Windoffer R, Leube R (2012). "Monitoring the Cytoskeletal EGF Response in Live Gastric Carcinoma Cells". PLOS ONE. DOI:10.1371/journal.pone.0045280. PMID 23028903. PMC: 3459943. | ||
Author | Felkl M, Tomas K, Smid M, Mattes J, Windoffer R, Leube R | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 16:37, 30 October 2012 | 7.7 s, 2,048 × 1,056 (21.43 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Felkl M, Tomas K, Smid M, Mattes J, Windoffer R, Leube R |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Ruffling activity is increased upon addition of EGF. MKN1 and Hs746T cells were transfected to produce enhanced yellow fluorescence protein (YFP) under CMV-promoter control. The fluorescence was recorded by confocal laser scanning microscopy three times per minute in the absence of EGF and after addition of 5 ng/ml EGF (corresponding Fig. 1A?B?). Note the EGF-induced cell spreading and the appearance of multiple ruffles and lamellipodia that leads to extensive lamella formation in MKN1 cells but not in Hs746T cells. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012 |