File:Molecular-and-Functional-Characterization-of-Hv1-Proton-Channel-in-Human-Granulocytes-pone.0014081.s005.ogv

English: Accumulation of Hv1 and Nox2 in the wall of a phagosome in a differentiated PLB-985 cell. Pseudo color, 3D projection calculated from a series of 1 µm thin confocal slices taken along the Z axis (perpendicular to the plane of the fibronectin coated coverslip to which the cell is attached). Green denotes aHv1-N labeling (AF 488), while 7D5 (Nox2) labeling is presented in red (AF 568). Engulfed zymosan particle can be recognized as labeling-free spherical structure on the cell periphery (see also Fig. 4, fourth column in the manuscript). Intense, overlapping clustering of the two signals can be observed in yellow. In the analysis only pixels with intensity at least two times the average intensity observed in experiments performed with control antibodies were included. Projection was performed to the brightest points, and pixels are interpolated.