File:Molecular-and-Functional-Characterization-of-Hv1-Proton-Channel-in-Human-Granulocytes-pone.0014081.s004.ogv

English: Accumulation of Hv1 and Nox2 in the wall of phagosomes in a neutrophil granulocyte. Pseudo color, 3D projection established from a series of 1 µm thin confocal slices taken along the Z axis (perpendicular to the plane of the fibronectin coated coverslip to which the cells are attached). Green denotes aHv1-N labeling (AF 488), while 7D5 (Nox2) labeling is presented in red (AF 568). Only the lower of the two neutrophils in contact displayed labeling with aHv1-N. The double labeled cell engulfed 3 zymosan particles (see also Fig. 4, third column in the manuscript). Zymosan particles can be recognized as labeling-free spherical structures inside the cell. Intense, overlapping clustering of the two signals (yellow) provides better outline of one of the particles. In the analysis only pixels with intensity at least the average intensity observed in experiments performed with control antibodies were included. Projection was performed to the brightest points and pixels are interpolated.