File:Mitotic-Spindle-Assembly-around-RCC1-Coated-Beads-in-Xenopus-Egg-Extracts-pbio.1001225.s006.ogv
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[edit]DescriptionMitotic-Spindle-Assembly-around-RCC1-Coated-Beads-in-Xenopus-Egg-Extracts-pbio.1001225.s006.ogv |
English: The effect of coupling kinesin 1 motor domain mutant R203K together with RCC1 to the bead. Two side-by-side fluorescence time-lapse movies of egg extract spindle assembly reactions containing rhodamine labeled tubulin and a bead coated either with RCC1 (left) or with RCC1 plus kinesin-1 motor domain ATPase mutant R203K, shown over the same time interval of 49 min. The non-motile kinesin stabilizes the RCC1 bead position within the center of the spindle, preventing bead oscillation (Quicktime, 1 MB). |
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Source | Video S4 from Halpin D, Kalab P, Wang J, Weis K, Heald R (2011). "Mitotic Spindle Assembly around RCC1-Coated Beads in Xenopus Egg Extracts". PLOS Biology. DOI:10.1371/journal.pbio.1001225. PMID 22215983. PMC: 3246454. | ||
Author | Halpin D, Kalab P, Wang J, Weis K, Heald R | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 01:53, 14 November 2012 | 6.7 s, 2,688 × 1,024 (1.26 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Halpin D, Kalab P, Wang J, Weis K, Heald R |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | The effect of coupling kinesin 1 motor domain mutant R203K together with RCC1 to the bead. Two side-by-side fluorescence time-lapse movies of egg extract spindle assembly reactions containing rhodamine labeled tubulin and a bead coated either with RCC1 (left) or with RCC1 plus kinesin-1 motor domain ATPase mutant R203K, shown over the same time interval of 49 min. The non-motile kinesin stabilizes the RCC1 bead position within the center of the spindle, preventing bead oscillation (Quicktime, 1 MB). |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2011-12 |