File:Mitochondrial-and-Plasma-Membrane-Pools-of-Stomatin-Like-Protein-2-Coalesce-at-the-Immunological-pone.0037144.s003.ogv

English: Stability of SLP-2 clusters during T cell activation. Jurkat T cells stably expressing SLP-2-gfp were incubated on polylysine-coated glass bottom microwell dishes for 10 minutes to allow cells to adhere. Adherent Jurkat T cells were then stimulated with OKT3 (1 µg/ml) for 15 minutes and then placed under confocal microscope. Forty images were acquired over a 375 seconds, with three images acquired before photobleaching. Area of cell indicated by arrow was photobleached using 100% of laser power for 3 seconds and the movement of SLP-2-gfp back into photobleached area was measured. Note that, in the stimulated T cell, it corresponds to an area with SLP-2-gfp clusters. Stimulated Jurkat T cells show little to no recovery of gfp signal in photobleached area within the examined time window.