File:Messages-Do-Diffuse-Faster-than-Messengers-Reconciling-Disparate-Estimates-of-the-Morphogen-Bicoid-pcbi.1003629.s006.ogv

English: FRAP. This video shows a simulated FRAP-like experiment for a system like the one probed in Video S2. In this simulation all the particles (free and bound) are assumed to be initially fluorescent and at equilibrium with the binding sites. The chemical parameters and rates are the same as in Video S2 (see Materials and Methods for more details). At , the particles in a spherical volume () which is in the center of the simulation volume are bleached. The simulated particles are diffusing in 3 dimensions but only two coordinates are shown. In this video we show all of the bleached particles (BLUE) and those unbleached particles (RED) that are inside the bleached volume. We observe how the fluorescence in the bleached volume recovers with time due to the diffusion of the free fluorescent particles. This recovery is quantified in Fig. 3.