File:Mechanical-Coupling-between-Endoderm-Invagination-and-Axis-Extension-in-Drosophila-pbio.1002292.s015.ogv

English: Example lateral view from mSPIM movie illustrating the temporal mapping of GBE (yellow), endoderm invagination (red), dorsal fold formation (green) and dorsal contraction (magenta). Lateral slice of reconstructed mSPIM movie of a wild-type embryo labeled with the whole-membrane markers resille-GFP and spider-GFP from 10 min before, to 10 min after the start of GBE. Anterior end is to the left, ventral side is to the bottom of the movie. Mapping of AP movement of individual ventral cells situated at the anterior and posterior of the germband (yellow dots), the two yellow cells initially move towards and then away from each other, the latter indicating the onset of GBE; mapping of apical constriction of two groups of cells at the posterior end of the embryo either side of the PC (red dots) indicating the onset of endoderm invagination; mapping of the apical surface of a dorsal cell in comparison to its initial position (green dots) on the dorsal surface indicating the initiation of the posterior dorsal fold; mapping of the movement of individual dorsal cells situated at the anterior and posterior of the dorsal side (magenta dots), cells get closer together indicating a dorsal contraction. See also Fig 4.