File:Mapping-the-dynamics-and-nanoscale-organization-of-synaptic-adhesion-proteins-using-monomeric-ncomms10773-s3.ogv
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[edit]DescriptionMapping-the-dynamics-and-nanoscale-organization-of-synaptic-adhesion-proteins-using-monomeric-ncomms10773-s3.ogv |
English: Supplementary Movie 2 Neuroligin-1 diffusion on the dendritic membrane and accumulation at synapses. DIV15 neurons co-expressing the synaptic marker Homer1c-GFP, AP-Nlg1, and BirAER were labeled with mSA-Atto 594, and single mSA molecules binding to AP-Nlg1 expressed at the cell surface were tracked by uPAINT. The movie represents 20 s (400 planes of 50 ms each). AP-Nlg1 is shown as red dots diffusing on the dendritic surface (dotted contour), and at synapses (white). Note the slow diffusion of Nlg1 at synapses. |
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Source | Video file from Chamma I, Letellier M, Butler C, Tessier B, Lim K, Gauthereau I, Choquet D, Sibarita J, Park S, Sainlos M, Thoumine O (2016). "Mapping the dynamics and nanoscale organization of synaptic adhesion proteins using monomeric streptavidin". Nature Communications. DOI:10.1038/ncomms10773. PMID 26979420. PMC: 4799371. | ||
Author | Chamma I, Letellier M, Butler C, Tessier B, Lim K, Gauthereau I, Choquet D, Sibarita J, Park S, Sainlos M, Thoumine O | ||
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![]() ![]() This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 17:07, 28 October 2016 | 20 s, 1,153 × 220 (1.26 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Supplementary Movie 2 |
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Author | Chamma I, Letellier M, Butler C, Tessier B, Lim K, Gauthereau I, Choquet D, Sibarita J, Park S, Sainlos M, Thoumine O |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Neuroligin-1 diffusion on the dendritic membrane and accumulation at synapses. DIV15 neurons co-expressing the synaptic marker Homer1c-GFP, AP-Nlg1, and BirAER were labeled with mSA-Atto 594, and single mSA molecules binding to AP-Nlg1 expressed at the cell surface were tracked by uPAINT. The movie represents 20 s (400 planes of 50 ms each). AP-Nlg1 is shown as red dots diffusing on the dendritic surface (dotted contour), and at synapses (white). Note the slow diffusion of Nlg1 at synapses. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2016-03-16 |