File:Mapping-the-dynamics-and-nanoscale-organization-of-synaptic-adhesion-proteins-using-monomeric-ncomms10773-s2.ogv

English: Supplementary Movie 1 mSA is washed off the cell surface by addition of free biotin. COS cells co-expressing GFP, AP-SEP-TfR, and BirAER were labeled with mSA-Atto 647N, and single mSA molecules bound to membrane-expressed AP-Nlg1 were tracked with uPAINT. A time lapse movie of 10 min was recorded at a rate of one image every 2 sec. 200 μM biotin was added after 2 min (white square). The movie shows the merge between the GFP image taken at the beginning of the recording (red), and single mSA-Atto 647N molecules tracked over time (green dots). Note the rapid decline in the number of mSA molecules after addition of biotin, reflecting their detachment from biotinylated AP-Nlg1 molecules by competition with excess free biotin.