File:MAL-Is-a-Regulator-of-the-Recruitment-of-Myelin-Protein-PLP-to-Membrane-Microdomains-pone.0155317.s003.ogv
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Size of this JPG preview of this OGG file: 555 × 600 pixels. Other resolutions: 222 × 240 pixels | 444 × 480 pixels | 685 × 740 pixels.
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[edit]DescriptionMAL-Is-a-Regulator-of-the-Recruitment-of-Myelin-Protein-PLP-to-Membrane-Microdomains-pone.0155317.s003.ogv |
English: Live cell imaging of PLP-eGFP in HepG2-PLP-MAL cells. The movement of PLP-eGFP-containing vesicles was monitored in live HepG2-PLP-MAL cells by time-lapse microscopy. Cells were recorded in the presence of cycloheximide for 1 hour at 37°C and 5% CO2. Images were taken every minute. The direction of 3 PLP-eGFP-containing vesicles in HepG2-PLP-MAL cells was tracked for 6 min each in Fiji using the manual tracker plugin. The video is played at 3 frames/sec; total time for shown image sequence is 11 min. |
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Source | S2 Movie from Bijlard M, de Jonge J, Klunder B, Nomden A, Hoekstra D, Baron W (2016). "MAL Is a Regulator of the Recruitment of Myelin Protein PLP to Membrane Microdomains". PLOS ONE. DOI:10.1371/journal.pone.0155317. PMID 27171274. PMC: 4865042. | ||
Author | Bijlard M, de Jonge J, Klunder B, Nomden A, Hoekstra D, Baron W | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 04:15, 13 October 2016 | 3.7 s, 685 × 740 (580 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Live cell imaging of PLP-eGFP in HepG2-PLP-MAL cells. |
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Author | Bijlard M, de Jonge J, Klunder B, Nomden A, Hoekstra D, Baron W |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | The movement of PLP-eGFP-containing vesicles was monitored in live HepG2-PLP-MAL cells by time-lapse microscopy. Cells were recorded in the presence of cycloheximide for 1 hour at 37°C and 5% CO2. Images were taken every minute. The direction of 3 PLP-eGFP-containing vesicles in HepG2-PLP-MAL cells was tracked for 6 min each in Fiji using the manual tracker plugin. The video is played at 3 frames/sec; total time for shown image sequence is 11 min. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2016-05-12 |
Categories:
- Videos of cell biology
- Cell membrane
- Videos of membrane proteins
- Biological transport
- Intracellular membranes
- Videos of physical sciences
- Videos of materials science
- Videos of developmental biology
- Cell differentiation
- Videos of physical chemistry
- Surface chemistry
- Videos of artificial membranes
- Media from PLOS ONE