File:Ménage-à-Trois-The-Ratio-of-Bicarbonate-to-CO2-and-the-pH-Regulate-the-Capacity-of-Neutrophils-to-Video 6.ogv
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[edit]DescriptionMénage-à-Trois-The-Ratio-of-Bicarbonate-to-CO2-and-the-pH-Regulate-the-Capacity-of-Neutrophils-to-Video 6.ogv |
English: The bicarbonate to CO2 ratio influences the neutrophil extracellular trap-inducing potential of monosodium urate crystals (MSU) in RPMI. Neutrophils were cultivated in RPMI containing either 0 mM (top) or 24 mM (bottom) bicarbonate in the copresence of 300 pg/ml MSU. Medium was supplied with 5% CO2. Cells were stained with 5 μM Sytox Green (green) and 0.05 μg/ml Hoechst 33342 (red). Hoechst 33342 (red) stains nuclei of cells with intact plasma membrane, whereas Sytox Green (green) only stains the nuclei of necrotic cells or extracellular DNA. Images were captured every 10 min for 240 min. Microscopy was performed on a BZ-X710 microscope using a time-lapse shooting sequence. Time is indicated in minutes and the scale bar represents 20 μm. |
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Source | Video S6 from Maueröder C, Mahajan A, Paulus S, Gößwein S, Hahn J, Kienhöfer D, Biermann M, Tripal P, Friedrich R, Munoz L, Neurath M, Becker C, Schett G, Herrmann M, Leppkes M (2016). "Ménage-à-Trois: The Ratio of Bicarbonate to CO2 and the pH Regulate the Capacity of Neutrophils to Form NETs". Frontiers in Immunology. DOI:10.3389/fimmu.2016.00583. PMID 28018350. PMC: 5145884. | ||
Author | Maueröder C, Mahajan A, Paulus S, Gößwein S, Hahn J, Kienhöfer D, Biermann M, Tripal P, Friedrich R, Munoz L, Neurath M, Becker C, Schett G, Herrmann M, Leppkes M | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 09:43, 1 February 2017 | 4.5 s, 1,756 × 2,304 (6.03 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Maueröder C, Mahajan A, Paulus S, Gößwein S, Hahn J, Kienhöfer D, Biermann M, Tripal P, Friedrich R, Munoz L, Neurath M, Becker C, Schett G, Herrmann M, Leppkes M |
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Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | The bicarbonate to CO2 ratio influences the neutrophil extracellular trap-inducing potential of monosodium urate crystals (MSU) in RPMI. Neutrophils were cultivated in RPMI containing either 0 mM (top) or 24 mM (bottom) bicarbonate in the copresence of 300 pg/ml MSU. Medium was supplied with 5% CO2. Cells were stained with 5 μM Sytox Green (green) and 0.05 μg/ml Hoechst 33342 (red). Hoechst 33342 (red) stains nuclei of cells with intact plasma membrane, whereas Sytox Green (green) only stains the nuclei of necrotic cells or extracellular DNA. Images were captured every 10 min for 240 min. Microscopy was performed on a BZ-X710 microscope using a time-lapse shooting sequence. Time is indicated in minutes and the scale bar represents 20 μm. |
User comments | Converted with https://clipchamp.com - online video converter, video compressor, and webcam recorder. Fast, reliable, and total privacy. |
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Date and time of digitizing | 2016-12-09 |
Language | English |