File:Ménage-à-Trois-The-Ratio-of-Bicarbonate-to-CO2-and-the-pH-Regulate-the-Capacity-of-Neutrophils-to-Video 5.ogv

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Original file (Ogg multiplexed audio/video file, Theora/Vorbis, length 8.1 s, 1,388 × 2,130 pixels, 1.11 Mbps overall, file size: 1.08 MB)

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English: Bicarbonate-induced formation of neutrophil extracellular traps is delayed by DIDS. Neutrophils were cultivated in isotonic HBSS medium containing 75 mM of bicarbonate and 1% of BSA either in the absence (top) or presence (bottom) of 0.1 mM DIDS. Cells were stained with 0.1 μg/ml Hoechst 33342 (green) and 500 ng/ml propidium iodide (red). Images were captured every 30 s for 30 min. Microscopy was performed on an Axio Observer.Z1 microscope using a time-lapse shooting sequence. Time is indicated in seconds and the scale bar represents 20 μm.
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Source Video S5 from Maueröder C, Mahajan A, Paulus S, Gößwein S, Hahn J, Kienhöfer D, Biermann M, Tripal P, Friedrich R, Munoz L, Neurath M, Becker C, Schett G, Herrmann M, Leppkes M (2016). "Ménage-à-Trois: The Ratio of Bicarbonate to CO2 and the pH Regulate the Capacity of Neutrophils to Form NETs". Frontiers in Immunology. DOI:10.3389/fimmu.2016.00583. PMID 28018350. PMC: 5145884.
Author Maueröder C, Mahajan A, Paulus S, Gößwein S, Hahn J, Kienhöfer D, Biermann M, Tripal P, Friedrich R, Munoz L, Neurath M, Becker C, Schett G, Herrmann M, Leppkes M
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Date/TimeThumbnailDimensionsUserComment
current09:42, 1 February 20178.1 s, 1,388 × 2,130 (1.08 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 1440P 540 kbps Completed 05:17, 4 September 2018 18 s
VP9 1080P 267 kbps Completed 05:17, 4 September 2018 10 s
VP9 720P 149 kbps Completed 05:17, 4 September 2018 8.0 s
VP9 480P 84 kbps Completed 05:16, 4 September 2018 6.0 s
VP9 360P 53 kbps Completed 05:16, 4 September 2018 4.0 s
VP9 240P 33 kbps Completed 05:16, 4 September 2018 4.0 s
WebM 360P 311 kbps Completed 09:43, 1 February 2017 6.0 s
QuickTime 144p (MJPEG) Not ready Unknown status

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