File:Ménage-à-Trois-The-Ratio-of-Bicarbonate-to-CO2-and-the-pH-Regulate-the-Capacity-of-Neutrophils-to-Video 2.ogv

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Original file (Ogg multiplexed audio/video file, Theora/Vorbis, length 4.5 s, 1,756 × 2,304 pixels, 14.54 Mbps overall, file size: 7.87 MB)

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English: High extracellular pH values induce formation of neutrophil extracellular traps in the absence of bicarbonate/CO2 in RPMI. Neutrophils were cultivated in RPMI containing 20 mM HEPES. pH was adjusted either to pH 6.6 (top) or pH 7.8 (bottom). Cells were stained with 5 μM Sytox Green (green). Images were captured every 10 min for 240 min. Microscopy was performed on a BZ-X710 microscope using a time-lapse shooting sequence. Time is indicated in minutes and the scale bar represents 20 μm.
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Source Video S2 from Maueröder C, Mahajan A, Paulus S, Gößwein S, Hahn J, Kienhöfer D, Biermann M, Tripal P, Friedrich R, Munoz L, Neurath M, Becker C, Schett G, Herrmann M, Leppkes M (2016). "Ménage-à-Trois: The Ratio of Bicarbonate to CO2 and the pH Regulate the Capacity of Neutrophils to Form NETs". Frontiers in Immunology. DOI:10.3389/fimmu.2016.00583. PMID 28018350. PMC: 5145884.
Author Maueröder C, Mahajan A, Paulus S, Gößwein S, Hahn J, Kienhöfer D, Biermann M, Tripal P, Friedrich R, Munoz L, Neurath M, Becker C, Schett G, Herrmann M, Leppkes M
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Date/TimeThumbnailDimensionsUserComment
current09:41, 1 February 20174.5 s, 1,756 × 2,304 (7.87 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 2160P 10.67 Mbps Completed 05:16, 4 September 2018 34 s
VP9 1440P 5.99 Mbps Completed 05:15, 4 September 2018 23 s
VP9 1080P 3.3 Mbps Completed 05:15, 4 September 2018 17 s
VP9 720P 1.58 Mbps Completed 05:15, 4 September 2018 13 s
VP9 480P 763 kbps Completed 05:15, 4 September 2018 10 s
VP9 360P 357 kbps Completed 05:15, 4 September 2018 10 s
VP9 240P 148 kbps Completed 05:15, 4 September 2018 3.0 s
WebM 360P 358 kbps Completed 09:42, 1 February 2017 4.0 s
QuickTime 144p (MJPEG) Not ready Unknown status

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