File:Longitudinal-Intravital-Imaging-of-the-Retina-Reveals-Long-term-Dynamics-of-Immune-Infiltration-and-Video 10.ogv
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[edit]DescriptionLongitudinal-Intravital-Imaging-of-the-Retina-Reveals-Long-term-Dynamics-of-Immune-Infiltration-and-Video 10.ogv |
English: Time-lapse intravital imaging of the retina of a healthy CERTN L15 mouse reveals neuronal calcium and, hence, neuronal function is not disturbed by the laser beam during multiphoton microscopy. Three-dimensional 1,400 μm × 1,400 μm × 300 μm FRET ratio images were acquired every minute, over 20 min in the retina of healthy CerTN L15 mice. In this mouse strain, some neuronal subsets express the TN L15 genetically encoded Ca2+ biosensor. The TN L15 biosensor is a FRET-biosensor based on Troponin C, a calcium-sensitive protein. The FRET pair contains Cerulean (cyan fluorescence) as donor and Citrine (yellow fluorescence) as acceptor. We used a three channel-based ratiometric FRET evaluation method to calculate the FRET ratio in the neurons and to evaluate the sustained neuronal calcium concentration. z-step = 6 μm. |
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Source | Video S10 from Bremer D, Pache F, Günther R, Hornow J, Andresen V, Leben R, Mothes R, Zimmermann H, Brandt A, Paul F, Hauser A, Radbruch H, Niesner R (2016). "Longitudinal Intravital Imaging of the Retina Reveals Long-term Dynamics of Immune Infiltration and Its Effects on the Glial Network in Experimental Autoimmune Uveoretinitis, without Evident Signs of Neuronal Dysfunction in the Ganglion Cell Layer". Frontiers in Immunology. DOI:10.3389/fimmu.2016.00642. PMID 28066446. PMC: 5179567. | ||
Author | Bremer D, Pache F, Günther R, Hornow J, Andresen V, Leben R, Mothes R, Zimmermann H, Brandt A, Paul F, Hauser A, Radbruch H, Niesner R | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 03:36, 1 February 2017 | 6.7 s, 994 × 994 (3.41 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Bremer D, Pache F, Günther R, Hornow J, Andresen V, Leben R, Mothes R, Zimmermann H, Brandt A, Paul F, Hauser A, Radbruch H, Niesner R |
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Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Time-lapse intravital imaging of the retina of a healthy CERTN L15 mouse reveals neuronal calcium and, hence, neuronal function is not disturbed by the laser beam during multiphoton microscopy. Three-dimensional 1,400 μm × 1,400 μm × 300 μm FRET ratio images were acquired every minute, over 20 min in the retina of healthy CerTN L15 mice. In this mouse strain, some neuronal subsets express the TN L15 genetically encoded Ca2+ biosensor. The TN L15 biosensor is a FRET-biosensor based on Troponin C, a calcium-sensitive protein. The FRET pair contains Cerulean (cyan fluorescence) as donor and Citrine (yellow fluorescence) as acceptor. We used a three channel-based ratiometric FRET evaluation method to calculate the FRET ratio in the neurons and to evaluate the sustained neuronal calcium concentration. z-step |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2016-12-23 |