File:Local-3D-matrix-microenvironment-regulates-cell-migration-through-spatiotemporal-dynamics-of-ncomms9720-s11.ogv
Local-3D-matrix-microenvironment-regulates-cell-migration-through-spatiotemporal-dynamics-of-ncomms9720-s11.ogv (Ogg multiplexed audio/video file, Theora/Vorbis, length 8.0 s, 640 × 480 pixels, 309 kbps overall, file size: 301 KB)
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[edit]DescriptionLocal-3D-matrix-microenvironment-regulates-cell-migration-through-spatiotemporal-dynamics-of-ncomms9720-s11.ogv |
English: Supplementary Movie 10 HFF expressing EYFP-paxillin (green or white) migrating through 3D FB16 collagen (red). Cell was treated with 25 μM blebbistatin and 1 μg ml-1 mAb13 to reduce β1 integrin binding. Inhibitors were added at the indicated time point. Protrusion initially occurs without cell body translocation. However, this is followed shortly by a release of the cell body, which slips through the ECM, rescuing the contractility-deficient condition. Stage was moved during imaging, and then realigned to track cells over long time periods. 5 minutes between frames. Total time 6 hours. |
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Source | Video file from Doyle A, Carvajal N, Jin A, Matsumoto K, Yamada K (2015). "Local 3D matrix microenvironment regulates cell migration through spatiotemporal dynamics of contractility-dependent adhesions". Nature Communications. DOI:10.1038/ncomms9720. PMID 26548801. PMC: 4643399. | ||
Author | Doyle A, Carvajal N, Jin A, Matsumoto K, Yamada K | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 10:40, 30 October 2016 | 8.0 s, 640 × 480 (301 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Supplementary Movie 10 |
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Author | Doyle A, Carvajal N, Jin A, Matsumoto K, Yamada K |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | HFF expressing EYFP-paxillin (green or white) migrating through 3D FB16 collagen (red). Cell was treated with 25 μM blebbistatin and 1 μg ml-1 mAb13 to reduce β1 integrin binding. Inhibitors were added at the indicated time point. Protrusion initially occurs without cell body translocation. However, this is followed shortly by a release of the cell body, which slips through the ECM, rescuing the contractility-deficient condition. Stage was moved during imaging, and then realigned to track cells over long time periods. 5 minutes between frames. Total time 6 hours. |
Software used | |
Date and time of digitizing | 2015-11-09 |