File:Live-cell-imaging-techniques-to-study-T-cell-trafficking-across-the-blood-brain-barrier-in-vitro-2045-8118-10-7-S4.ogv
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Live-cell-imaging-techniques-to-study-T-cell-trafficking-across-the-blood-brain-barrier-in-vitro-2045-8118-10-7-S4.ogv (Ogg Theora video file, length 21 s, 352 × 288 pixels, 567 kbps, file size: 1.42 MB)
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[edit]DescriptionLive-cell-imaging-techniques-to-study-T-cell-trafficking-across-the-blood-brain-barrier-in-vitro-2045-8118-10-7-S4.ogv |
English: Movie 4. Initial contact of CD8+ T cells with the spinal cord microvasculature during EAE. At the beginning, the inflamed spinal cord microvasculature is visualized by TRITC-Dextran in the circulation of a C57/BL6 mouse affected with EAE within one field of view (FOV) at x10 objective. The spinal cord white matter microvasculature is visualized above the large spinal cord collecting vein, always seen at the bottom of each video frame. After switching the fluorescence filter on the microscope, the infusion of 1 aliquot (1.3 x 106 cells/100μL) of CellTracker green CD8+ T cells, pretreated for 20 min with rat IgG isotype control, is started and T cells can be observed either passing through the corresponding vascular beds or interacting as rolling or capturing to the microvasculature in real time (objective x10) within one FOV. |
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Source | Coisne C, Lyck R, Engelhardt B (2013). "Live cell imaging techniques to study T cell trafficking across the blood-brain barrier in vitro and in vivo". Fluids and Barriers of the CNS. DOI:10.1186/2045-8118-10-7. PMID 23336847. PMC: 3560242. | ||
Author | Coisne C, Lyck R, Engelhardt B | ||
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This file is licensed under the Creative Commons Attribution 2.0 Generic license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 21:31, 9 February 2013 | 21 s, 352 × 288 (1.42 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Additional file 4 |
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Author | Coisne C, Lyck R, Engelhardt B |
Usage terms | http://creativecommons.org/licenses/by/2.0/ |
Image title | Movie 4. Initial contact of CD8+ T cells with the spinal cord microvasculature during EAE. At the beginning, the inflamed spinal cord microvasculature is visualized by TRITC-Dextran in the circulation of a C57/BL6 mouse affected with EAE within one field of view (FOV) at x10 objective. The spinal cord white matter microvasculature is visualized above the large spinal cord collecting vein, always seen at the bottom of each video frame. After switching the fluorescence filter on the microscope, the infusion of 1 aliquot (1.3 x 106 cells/100?L) of CellTracker green CD8+ T cells, pretreated for 20?min with rat IgG isotype control, is started and T cells can be observed either passing through the corresponding vascular beds or interacting as rolling or capturing to the microvasculature in real time (objective x10) within one FOV. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013 |