File:Live-Imaging-of-Innate-Immune-Cell-Sensing-of-Transformed-Cells-in-Zebrafish-Larvae-Parallels-pbio.1000562.s007.ogv
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[edit]DescriptionLive-Imaging-of-Innate-Immune-Cell-Sensing-of-Transformed-Cells-in-Zebrafish-Larvae-Parallels-pbio.1000562.s007.ogv |
English: Supporting movies for Figure 3. (A) is a time-lapse movie made from a Fli:eGFP/kita:GalTA4/UAS:V12RASeGFP+ larva that shows the earliest recognition of V12RAS-transformed cells by Fli:eGFP+ leukocytes at the initial stage of transformed cell expansion. Green arrows indicate V12RAS+ mucus-secreting cells. These cells express V12RASeGFP and so GFP appears membrane-located in these cells. Leukocytes are motile and labeled by Fli:eGFP, and the GFP signal in these cells has a pale cytoplasmic distribution. (B) shows an example of how LysC:DsRed+ leukocytes respond to V12RAS+ clones at their “early developmental” stage. (C) shows an example of how LysC:DsRed+ cells respond to a “late developmental” stage V12RAS+ clone. Note how LysC:DsRed+ cells stay with the clone for a prolonged period. |
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Source | Video S5 from Feng Y, Santoriello C, Mione M, Hurlstone A, Martin P (2010). "Live Imaging of Innate Immune Cell Sensing of Transformed Cells in Zebrafish Larvae: Parallels between Tumor Initiation and Wound Inflammation". PLOS Biology. DOI:10.1371/journal.pbio.1000562. PMID 21179501. PMC: 3001901. | ||
Author | Feng Y, Santoriello C, Mione M, Hurlstone A, Martin P | ||
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current | 00:49, 31 October 2012 | 18 s, 1,536 × 512 (3.37 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Feng Y, Santoriello C, Mione M, Hurlstone A, Martin P |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Supporting movies for Figure 3. (A) is a time-lapse movie made from a Fli:eGFP/kita:GalTA4/UAS:V12RASeGFP+ larva that shows the earliest recognition of V12RAS-transformed cells by Fli:eGFP+ leukocytes at the initial stage of transformed cell expansion. Green arrows indicate V12RAS+ mucus-secreting cells. These cells express V12RASeGFP and so GFP appears membrane-located in these cells. Leukocytes are motile and labeled by Fli:eGFP, and the GFP signal in these cells has a pale cytoplasmic distribution. (B) shows an example of how LysC:DsRed+ leukocytes respond to V12RAS+ clones at their ?early developmental? stage. (C) shows an example of how LysC:DsRed+ cells respond to a ?late developmental? stage V12RAS+ clone. Note how LysC:DsRed+ cells stay with the clone for a prolonged period. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2010-12 |
Categories:
- Molecular development
- Videos of disease models
- Innate immunity
- Leukocyte activation
- Videos of neoplasms of the digestive system
- Videos of skin cancers
- Immune response
- Dysplasias
- Animal models of disease
- Gene knockdown techniques
- Larvae
- Videos of melanocytes
- Videos of melanoma
- Confocal microscopic movies from Open Access Journals
- Videos of morpholines
- NADPH oxidase
- Proto-oncogene proteins p21
- Videos of wounds
- Videos of Zebrafish
- Zebrafish proteins
- Red fluorescent protein (fp583 or dsred(clontech))