File:Lentiviral-Vectors-and-Protocols-for-Creation-of-Stable-hESC-Lines-for-Fluorescent-Tracking-and-pone.0005046.s013.ogv
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Lentiviral-Vectors-and-Protocols-for-Creation-of-Stable-hESC-Lines-for-Fluorescent-Tracking-and-pone.0005046.s013.ogv (Ogg Theora video file, length 7.7 s, 450 × 450 pixels, 433 kbps, file size: 404 KB)
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[edit]DescriptionLentiviral-Vectors-and-Protocols-for-Creation-of-Stable-hESC-Lines-for-Fluorescent-Tracking-and-pone.0005046.s013.ogv |
English: Example of cardiomyocytes labeled with αMHC-mCherry. The hESC line was engineered with αMHC-Puror_Rex-Neor and αMHC-mCherry_Rex-Blar vectors (Figure 1A) by G418 and Blasticidin selection (Methods). Phase contrast and fluorescence images show mCherry+ cardiomyocytes within the EB plated on gelatin-coated cell culture plastic without Puromycin treatment. |
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Source | Movie S3 from Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot J, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R, Calzolari D, Terskikh A, McCulloch A, Price J, Conklin B, Chen H, Mercola M (2009). "Lentiviral Vectors and Protocols for Creation of Stable hESC Lines for Fluorescent Tracking and Drug Resistance Selection of Cardiomyocytes". PLOS ONE. DOI:10.1371/journal.pone.0005046. PMID 19352491. PMC: 2662416. | ||
Author | Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot J, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R, Calzolari D, Terskikh A, McCulloch A, Price J, Conklin B, Chen H, Mercola M | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 08:08, 31 October 2012 | 7.7 s, 450 × 450 (404 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot J, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R, Calzolari D, Terskikh A, McCulloch A, Price J, Conklin B, Chen H, Mercola M |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Example of cardiomyocytes labeled with ?MHC-mCherry. The hESC line was engineered with ?MHC-Puror_Rex-Neor and ?MHC-mCherry_Rex-Blar vectors (Figure 1A) by G418 and Blasticidin selection (Methods). Phase contrast and fluorescence images show mCherry+ cardiomyocytes within the EB plated on gelatin-coated cell culture plastic without Puromycin treatment. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2009 |
Categories:
- Videos of cell differentiation
- Base sequence
- DNA primers
- Videos of drug resistance
- Videos of embryonic stem cells
- Fetal proteins
- Flow cytometry
- Gene expression profiling
- Genetic vectors
- Videos of green fluorescent proteins
- Lentivirus
- Videos of myocardium
- Genetic promoter regions
- Reverse transcriptase polymerase chain reaction
- T-box domain proteins
- Media from PLOS ONE
- MCherry