File:Lentiviral-Vectors-and-Protocols-for-Creation-of-Stable-hESC-Lines-for-Fluorescent-Tracking-and-pone.0005046.s012.ogv
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Lentiviral-Vectors-and-Protocols-for-Creation-of-Stable-hESC-Lines-for-Fluorescent-Tracking-and-pone.0005046.s012.ogv (Ogg Theora video file, length 8.5 s, 592 × 216 pixels, 234 kbps, file size: 243 KB)
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[edit]DescriptionLentiviral-Vectors-and-Protocols-for-Creation-of-Stable-hESC-Lines-for-Fluorescent-Tracking-and-pone.0005046.s012.ogv |
English: Example of cell tracking using PGK-H2BmCherry hESCs. Time-lapse image stacks of PGK-H2BmCherry ESCs over 20 hours were acquired with a 10× objective (NA 0.5) at 10 minute intervals using MetaMorph. Centroids of H2BmCherry fluorescence were tracked automatically using a modified version of the Particle Tracking Plugin [17] for ImageJ (Methods). The 4 concatenated clips correspond to single tracks within the yellow boxes in Figure 2C. |
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Source | Movie S2 from Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot J, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R, Calzolari D, Terskikh A, McCulloch A, Price J, Conklin B, Chen H, Mercola M (2009). "Lentiviral Vectors and Protocols for Creation of Stable hESC Lines for Fluorescent Tracking and Drug Resistance Selection of Cardiomyocytes". PLOS ONE. DOI:10.1371/journal.pone.0005046. PMID 19352491. PMC: 2662416. | ||
Author | Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot J, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R, Calzolari D, Terskikh A, McCulloch A, Price J, Conklin B, Chen H, Mercola M | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 08:07, 31 October 2012 | 8.5 s, 592 × 216 (243 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot J, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R, Calzolari D, Terskikh A, McCulloch A, Price J, Conklin B, Chen H, Mercola M |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2009 |
Categories:
- Videos of cell differentiation
- Base sequence
- DNA primers
- Videos of drug resistance
- Videos of embryonic stem cells
- Fetal proteins
- Flow cytometry
- Gene expression profiling
- Genetic vectors
- Videos of green fluorescent proteins
- Lentivirus
- Videos of myocardium
- Genetic promoter regions
- Reverse transcriptase polymerase chain reaction
- T-box domain proteins
- Media from PLOS ONE