File:Lentiviral-Vectors-and-Protocols-for-Creation-of-Stable-hESC-Lines-for-Fluorescent-Tracking-and-pone.0005046.s012.ogv

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Lentiviral-Vectors-and-Protocols-for-Creation-of-Stable-hESC-Lines-for-Fluorescent-Tracking-and-pone.0005046.s012.ogv ‎(Ogg Theora video file, length 8.5 s, 592 × 216 pixels, 234 kbps, file size: 243 KB)

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English: Example of cell tracking using PGK-H2BmCherry hESCs. Time-lapse image stacks of PGK-H2BmCherry ESCs over 20 hours were acquired with a 10× objective (NA 0.5) at 10 minute intervals using MetaMorph. Centroids of H2BmCherry fluorescence were tracked automatically using a modified version of the Particle Tracking Plugin [17] for ImageJ (Methods). The 4 concatenated clips correspond to single tracks within the yellow boxes in Figure 2C.
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Source Movie S2 from Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot J, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R, Calzolari D, Terskikh A, McCulloch A, Price J, Conklin B, Chen H, Mercola M (2009). "Lentiviral Vectors and Protocols for Creation of Stable hESC Lines for Fluorescent Tracking and Drug Resistance Selection of Cardiomyocytes". PLOS ONE. DOI:10.1371/journal.pone.0005046. PMID 19352491. PMC: 2662416.
Author Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot J, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R, Calzolari D, Terskikh A, McCulloch A, Price J, Conklin B, Chen H, Mercola M
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current08:07, 31 October 20128.5 s, 592 × 216 (243 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 68 kbps Completed 17:29, 1 September 2018 2.0 s
Streaming 240p (VP9) 68 kbps Completed 00:10, 15 January 2024 1.0 s
WebM 360P 114 kbps Completed 20:33, 1 December 2023 1.0 s
Streaming 144p (MJPEG) 159 kbps Completed 17:18, 9 November 2023 1.0 s

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