File:Laser-surgery-of-zebrafish-(Danio-rerio)-embryos-using-femtosecond-laser-pulses-Optimal-parameters-1472-6750-8-7-S1.ogv
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Laser-surgery-of-zebrafish-(Danio-rerio)-embryos-using-femtosecond-laser-pulses-Optimal-parameters-1472-6750-8-7-S1.ogv (Ogg Theora video file, length 56 s, 320 × 240 pixels, 319 kbps, file size: 2.14 MB)
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[edit]DescriptionLaser-surgery-of-zebrafish-(Danio-rerio)-embryos-using-femtosecond-laser-pulses-Optimal-parameters-1472-6750-8-7-S1.ogv |
English: Additional file 1
Video demonstrating surgical-ablation of a 2-cell stage chorionated embryo. First video sequence depicts the 2-cell stage chorionated embryo at 4× and 60× magnification. Second video sequence shows ablation using an average laser power of 45 mW with a beam dwell time of 100 ms and the galvo pulsed a total of 3 times. Third video sequence depicts ablation of the same embryo at a different location using an average laser power of 45 mW with a beam dwell time of 500 ms and a total of 2 galvo pulsings. Ablation was performed near the blastomere-yolk interface using a 1.0 NA water immersion microscope objective. |
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Source | Kohli V, Elezzabi A. "Laser surgery of zebrafish (Danio rerio) embryos using femtosecond laser pulses: Optimal parameters for exogenous material delivery, and the laser's effect on short- and long-term development". BMC Biotechnology. DOI:10.1186/1472-6750-8-7. PMID 18230185. PMC: 2270268. | ||
Author | Kohli V, Elezzabi A | ||
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![]() ![]() This file is licensed under the Creative Commons Attribution 2.0 Generic license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 21:10, 20 October 2012 | 56 s, 320 × 240 (2.14 MB) | Open Access Media Importer Bot (talk | contribs) | Uploaded with the Open Access Media Importer. (test edit) botrequest |
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Short title | Additional file 1 |
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Author | Kohli V, Elezzabi A |
Usage terms | http://creativecommons.org/licenses/by/2.0/ |
Image title | |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2008-01-29 |