File:Killing-Hypoxic-Cell-Populations-in-a-3D-Tumor-Model-with-EtNBS-PDT-pone.0023434.s003.ogv

English: Time-lapse optical coherence tomography (TL-OCT) of the treatment response of day 13 nodules following EtNBS-PDT. Each frame of the move is a single xz cross-sectional image through three OvCa nodules. A fraction of the entire depth profile through the Matrigel is shown. The three nodules are seen to sit in the Matrigel bed, and the movie starts immediately following EtNBS-PDT. The nodules are observed to burst open from the inside, rapidly filling with highly scattering apoptotic bodies within five hours following treatment. Cells continue to apoptose over the next several hours, and apoptotic bodies are observed spilling out onto the Matrigel surface. Shaking of the thick Matrigel bed during image acquisition causes the small amount of image warping in the movie. The opening and spilling process seen here explains why nodules treated with EtNBS-PDT at high fluences appear larger than nodules treated at low fluences when viewed with a confocal microscope (Figure 7): by spilling out on the Matrigel bed, the dying nodule spreads out over the gel surface. The movie dimensions are 2.13 mm×0.678 mm.