File:K+-Channel-Inhibition-Differentially-Regulates-Migration-of-Intestinal-Epithelial-Cells-in-Inflamed-pone.0147736.s006.ogv

English: Restitution of scratch-wounded IEC-18 monolayers. A confluent IEC-18 monolayer was mechanically wounded and imaged every 15 minutes for six hours. A video sequence was composed using MAGIX Video (Berlin, Germany) and is repeated several times to demonstrate different issues. A: Representative time lapse video of epithelial restitution. B: Cells located at the wound margin migrate into the denuded area protruding filopodia (red arrows) and pseudopodia (orange arrows). C: Five representative cells directly adjacent to the wound are edged in red. They migrate into the denuded area undergoing profound morphological changes. D: Five representative cells closely but not directly neighboring the wound are edged in red. They also engage in wound closure by migrating behind the first cell line, therefore showing properties of ‘collective sheet migration’. E: Five representative cells distant from the wound are edged in red. Over the time, they barely move nor do they considerably change shape.