File:Involvement-of-Local-Lamellipodia-in-Endothelial-Barrier-Function-pone.0117970.s013.ogv
Involvement-of-Local-Lamellipodia-in-Endothelial-Barrier-Function-pone.0117970.s013.ogv (Ogg Theora video file, length 15 s, 696 × 520 pixels, 3.94 Mbps, file size: 7.05 MB)
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[edit]DescriptionInvolvement-of-Local-Lamellipodia-in-Endothelial-Barrier-Function-pone.0117970.s013.ogv |
English: Impact of 1 U/ml thrombin treatment on dynamics of the GFP-actin cytoskeleton in endothelial cells. This movie is a continuation of the same cells shown in S1 Movie, which were treated with thrombin at the 60 min time point. Shortly after thrombin treatment, actin appears at the cell edges and on vesicles. Soon after, stress fibers assemble in the cytoplasm and transverse arc fibers moved inward. A gap at the junction of three cells also opens and closes. Elapsed time is shown in the bottom right corner. Due to fading of the GFP intensity over the time course of S1 Movie, the brightness and contrast were readjusted to optimize view of the GFP-actin structures. |
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Source | S2 Movie from Breslin J, Zhang X, Worthylake R, Souza-Smith F (2015). "Involvement of Local Lamellipodia in Endothelial Barrier Function". PLOS ONE. DOI:10.1371/journal.pone.0117970. PMID 25658915. PMC: 4320108. | ||
Author | Breslin J, Zhang X, Worthylake R, Souza-Smith F | ||
Permission (Reusing this file) |
![]() ![]() This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 01:46, 20 February 2015 | 15 s, 696 × 520 (7.05 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Impact of 1 U/ml thrombin treatment on dynamics of the GFP-actin cytoskeleton in endothelial cells. |
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Author | Breslin J, Zhang X, Worthylake R, Souza-Smith F |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | This movie is a continuation of the same cells shown in S1 Movie, which were treated with thrombin at the 60 min time point. Shortly after thrombin treatment, actin appears at the cell edges and on vesicles. Soon after, stress fibers assemble in the cytoplasm and transverse arc fibers moved inward. A gap at the junction of three cells also opens and closes. Elapsed time is shown in the bottom right corner. Due to fading of the GFP intensity over the time course of S1 Movie, the brightness and contrast were readjusted to optimize view of the GFP-actin structures. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2015-02-06 |