File:Intoxication-of-Host-Cells-by-the-T3SS-Phospholipase-ExoU-PI(45)P2-Associated-Cytoskeletal-Collapse-pone.0103127.s006.ogv
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Intoxication-of-Host-Cells-by-the-T3SS-Phospholipase-ExoU-PI(45)P2-Associated-Cytoskeletal-Collapse-pone.0103127.s006.ogv (Ogg Theora video file, length 16 s, 400 × 246 pixels, 352 kbps, file size: 687 KB)
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[edit]DescriptionIntoxication-of-Host-Cells-by-the-T3SS-Phospholipase-ExoU-PI(45)P2-Associated-Cytoskeletal-Collapse-pone.0103127.s006.ogv |
English: Lyso-phospholipid-induced cell death. The addition of 20 µM lyso-PC, a product of PLA2 enzymatic activity, intoxicated cells without cell rounding, nuclear shrinkage, or the membrane blebbing phenotype. The nucleus and cell swelled upon cell lysis, which is characteristic of necrosis. Cells were visualized by staining with SYTOX green, CellMask plasma membrane stain (red), Hoechst 33342 (blue). The images were acquired by time-lapse microscopy for 40 min with 30 sec interval. |
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Source | Movie S5 from Sato H, Frank D (2014). "Intoxication of Host Cells by the T3SS Phospholipase ExoU: PI(4,5)P2-Associated, Cytoskeletal Collapse and Late Phase Membrane Blebbing". PLOS ONE. DOI:10.1371/journal.pone.0103127. PMID 25061861. PMC: 4111512. | ||
Author | Sato H, Frank D | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 20:12, 30 July 2014 | 16 s, 400 × 246 (687 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Sato H, Frank D |
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Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Lyso-phospholipid-induced cell death. The addition of 20 µM lyso-PC, a product of PLA2 enzymatic activity, intoxicated cells without cell rounding, nuclear shrinkage, or the membrane blebbing phenotype. The nucleus and cell swelled upon cell lysis, which is characteristic of necrosis. Cells were visualized by staining with SYTOX green, CellMask plasma membrane stain (red), Hoechst 33342 (blue). The images were acquired by time-lapse microscopy for 40 min with 30 sec interval. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2014 |