File:Inter-Cellular-Exchange-of-Cellular-Components-via-VE-Cadherin-Dependent-Trans-Endocytosis-pone.0090736.s007.ogv
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Inter-Cellular-Exchange-of-Cellular-Components-via-VE-Cadherin-Dependent-Trans-Endocytosis-pone.0090736.s007.ogv (Ogg Theora video file, length 12 s, 728 × 238 pixels, 1.05 Mbps, file size: 1.45 MB)
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[edit]DescriptionInter-Cellular-Exchange-of-Cellular-Components-via-VE-Cadherin-Dependent-Trans-Endocytosis-pone.0090736.s007.ogv |
English: Time-lapse imaging of co-culture of COS7 cells expressing VEC-EGFP and HUVECs expressing VEC-TagRFP657. The interaction between endogenous VEC in HUVECs and over-expressed VEC-EGFP in COS7 cells can induce trans-endocytosis. The trans-endocytosed VEC-EGFP molecules from a COS7 cell appeared to bud off from cell-cell junctions and be pulled into a HUVEC. The time-lapse images were acquired with a 60x objective lens every 13 seconds for 25 minutes by spinning disk confocal microscopy and were played back as movie at 10 frames per second. |
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Source | Movie S1 from Sakurai T, Woolls M, Jin S, Murakami M, Simons M (2014). "Inter-Cellular Exchange of Cellular Components via VE-Cadherin-Dependent Trans-Endocytosis". PLOS ONE. DOI:10.1371/journal.pone.0090736. PMID 24603875. PMC: 3946293. | ||
Author | Sakurai T, Woolls M, Jin S, Murakami M, Simons M | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 07:33, 15 March 2014 | 12 s, 728 × 238 (1.45 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Sakurai T, Woolls M, Jin S, Murakami M, Simons M |
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Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Time-lapse imaging of co-culture of COS7 cells expressing VEC-EGFP and HUVECs expressing VEC-TagRFP657. The interaction between endogenous VEC in HUVECs and over-expressed VEC-EGFP in COS7 cells can induce trans-endocytosis. The trans-endocytosed VEC-EGFP molecules from a COS7 cell appeared to bud off from cell-cell junctions and be pulled into a HUVEC. The time-lapse images were acquired with a 60x objective lens every 13 seconds for 25 minutes by spinning disk confocal microscopy and were played back as movie at 10 frames per second. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2014 |