File:Insulin-Regulates-Glut4-Confinement-in-Plasma-Membrane-Clusters-in-Adipose-Cells-pone.0057559.s011.ogv
From Wikimedia Commons, the free media repository
Jump to navigation
Jump to search
No higher resolution available.
Insulin-Regulates-Glut4-Confinement-in-Plasma-Membrane-Clusters-in-Adipose-Cells-pone.0057559.s011.ogv (Ogg Theora video file, length 6.9 s, 372 × 124 pixels, 1.68 Mbps, file size: 1.37 MB)
File information
Structured data
Captions
Captions
Add a one-line explanation of what this file represents
Summary
[edit]DescriptionInsulin-Regulates-Glut4-Confinement-in-Plasma-Membrane-Clusters-in-Adipose-Cells-pone.0057559.s011.ogv |
English: Example of “fusion-with-retention” - GLUT4 vesicle fusion resulting in selective retention of GLUT4 molecules and cluster formation at the site of the fusion. Cells were co-transfected with HA-GLUT4-EOS and IRAP-pHluorin and imaged 24 h after transfection. To monitor post-fusion dispersal of individual GLUT4 molecules, cells were first illuminated with 15–20 consecutive pulses of 405 nm laser at a wide-field illumination mode to achieve near complete photoconversion of HA-GLUT4-EOS into red-fluorescent state in PM and intracellular structures. Then cells were illuminated with a 561 nm laser in TIRF mode to selectively bleach HA-GLUT4-EOS molecules present at the PM. Red channel corresponds to activated HA-GLUT4-EOS molecules; green channel corresponds to Irap-pHluorin fluorescence. Irap-pHluorin fluorescence spike corresponds to luminal pH equilibration upon fusion pore opening. Scale bar, 2 µm. |
||
Date | |||
Source | Movie S8 from Lizunov V, Stenkula K, Troy A, Cushman S, Zimmerberg J (2013). "Insulin Regulates Glut4 Confinement in Plasma Membrane Clusters in Adipose Cells". PLOS ONE. DOI:10.1371/journal.pone.0057559. PMID 23520472. PMC: 3592853. | ||
Author | Lizunov V, Stenkula K, Troy A, Cushman S, Zimmerberg J | ||
Permission (Reusing this file) |
|
||
Provenance InfoField |
|
File history
Click on a date/time to view the file as it appeared at that time.
Date/Time | Thumbnail | Dimensions | User | Comment | |
---|---|---|---|---|---|
current | 19:44, 3 June 2013 | 6.9 s, 372 × 124 (1.37 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
You cannot overwrite this file.
File usage on Commons
There are no pages that use this file.
Transcode status
Update transcode statusMetadata
This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.
Author | Lizunov V, Stenkula K, Troy A, Cushman S, Zimmerberg J |
---|---|
Usage terms | http://creativecommons.org/publicdomain/zero/1.0/ |
Image title | |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013 |