File:Imaging-plant-cell-death-GFP-Nit1-aggregation-marks-an-early-step-of-wound-and-herbicide-induced-1471-2229-5-4-S1.ogv

English: Additional File 1 Wound induced granulation of GFP-Nit1 demonstrating action at a distance A 35S GFP-Nit1 root was wounded above the root meristem with a syringe tip, quickly aligned for microscopy and then imaged at a single focal plane at 4 second intervals for 8 minutes (the wound site is out of the focal plane of the time series, but is visible as the dark area of separated cells in the center of the root tip). Initially, the GFP-Nit1marker is visible as diffuse fluorescence. Over the course of several minutes, bright granular structures become evident concomitant with a decrease in cytoplasmic fluorescence, suggesting a redistribution of the marker into granules. The total time of the image series is 8 minutes. In other time series which have imaged the process over a longer time period, we have observed reversion of the marker to cytoplasmic distribution with resumption of cytoplasmic streaming (see additional file3). The action-at-a distance observed with this marker suggests that the marker is responsive to a motile signal is sent after wounding. The data shown in figure1A were derived from this data set.