File:Identification-of-Contractile-Vacuole-Proteins-in-Trypanosoma-cruzi-pone.0018013.s011.ogv
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Identification-of-Contractile-Vacuole-Proteins-in-Trypanosoma-cruzi-pone.0018013.s011.ogv (Ogg Theora video file, length 10 s, 518 × 548 pixels, 19 kbps, file size: 24 KB)
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[edit]DescriptionIdentification-of-Contractile-Vacuole-Proteins-in-Trypanosoma-cruzi-pone.0018013.s011.ogv |
English: Rab11 and BODIPY-ceramide co-localization. Images of labeled cells (Fig. 4C) were captured in the channels blue, red and green by Z-sectioning in order to obtain 25 optical sectioned images with 0.2 µm of optical section space between each one, covering 5 µm of sample thickness. Images were then deconvolved using the softWorx toolbar. Deconvolved sections were grouped to give a volume perspective of the labeling using the volume viewer tool (softWorx tool bar) and finally saved as a movie showing a rotation of 180 degrees around the Y axis. Imunofluorescence techniques are described in material & methods section. Scale bar = 10 µm. |
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Source | Movie S1 from Ulrich P, Jimenez V, Park M, Martins V, Atwood J, Moles K, Collins D, Rohloff P, Tarleton R, Moreno S, Orlando R, Docampo R (2011). "Identification of Contractile Vacuole Proteins in Trypanosoma cruzi". PLOS ONE. DOI:10.1371/journal.pone.0018013. PMID 21437209. PMC: 3060929. | ||
Author | Ulrich P, Jimenez V, Park M, Martins V, Atwood J, Moles K, Collins D, Rohloff P, Tarleton R, Moreno S, Orlando R, Docampo R | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 22:17, 28 May 2013 | 10 s, 518 × 548 (24 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Ulrich P, Jimenez V, Park M, Martins V, Atwood J, Moles K, Collins D, Rohloff P, Tarleton R, Moreno S, Orlando R, Docampo R |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2011 |
Categories:
- Videos of transmembrane proteins
- Parasite physiology
- Videos of protista
- Western blot
- Contractile proteins
- Videos of green fluorescent proteins
- Videos of fluorescence microscopy
- Protein transport
- Protozoan proteins
- Recombinant fusion proteins
- Trypanosoma cruzi
- Vacuolar proton-translocating ATPases
- Media from PLOS ONE