File:ICP0-Dismantles-Microtubule-Networks-in-Herpes-Simplex-Virus-Infected-Cells-pone.0010975.s008.ogv

English: Bundles of ICP0ΔRING disperse following nocodazole treatment of HSV-1 0ΔRING-infected cells. Vero cells were inoculated with 5 pfu per cell of HSV-1 0ΔRING in the presence of 200 µM cycloheximide from −0.5 to 10 hours p.i., and were released into medium containing no drugs. At 4.0 hours post-release, a field of cells was chosen and a 3× solution of nocodazole was added to culture medium to achieve a final concentration of 10 µg/ml. Time-lapse photography of ICP0ΔRING protein was commenced 1 minute later. Cultures were continuously incubated at 37°C before and during time-lapse photography. Photographs were captured at a rate of twice per minute for 30 minutes, and are shown in this video at an elapsed rate that is 150 times faster than normal.