File:ICP0-Dismantles-Microtubule-Networks-in-Herpes-Simplex-Virus-Infected-Cells-pone.0010975.s008.ogv
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ICP0-Dismantles-Microtubule-Networks-in-Herpes-Simplex-Virus-Infected-Cells-pone.0010975.s008.ogv (Ogg Theora video file, length 12 s, 300 × 300 pixels, 168 kbps, file size: 250 KB)
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[edit]DescriptionICP0-Dismantles-Microtubule-Networks-in-Herpes-Simplex-Virus-Infected-Cells-pone.0010975.s008.ogv |
English: Bundles of ICP0ΔRING disperse following nocodazole treatment of HSV-1 0ΔRING-infected cells. Vero cells were inoculated with 5 pfu per cell of HSV-1 0ΔRING in the presence of 200 µM cycloheximide from −0.5 to 10 hours p.i., and were released into medium containing no drugs. At 4.0 hours post-release, a field of cells was chosen and a 3× solution of nocodazole was added to culture medium to achieve a final concentration of 10 µg/ml. Time-lapse photography of ICP0ΔRING protein was commenced 1 minute later. Cultures were continuously incubated at 37°C before and during time-lapse photography. Photographs were captured at a rate of twice per minute for 30 minutes, and are shown in this video at an elapsed rate that is 150 times faster than normal. |
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Source | Video S4 from Liu M, Schmidt E, Halford W (2010). "ICP0 Dismantles Microtubule Networks in Herpes Simplex Virus-Infected Cells". PLOS ONE. DOI:10.1371/journal.pone.0010975. PMID 20544015. PMC: 2882321. | ||
Author | Liu M, Schmidt E, Halford W | ||
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This file is licensed under the Creative Commons Attribution 3.0 Unported license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 17:32, 16 October 2016 | 12 s, 300 × 300 (250 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Liu M, Schmidt E, Halford W |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Bundles of ICP0ΔRING disperse following nocodazole treatment of HSV-1 0ΔRING-infected cells. Vero cells were inoculated with 5 pfu per cell of HSV-1 0ΔRING in the presence of 200 µM cycloheximide from −0.5 to 10 hours p.i., and were released into medium containing no drugs. At 4.0 hours post-release, a field of cells was chosen and a 3× solution of nocodazole was added to culture medium to achieve a final concentration of 10 µg/ml. Time-lapse photography of ICP0ΔRING protein was commenced 1 minute later. Cultures were continuously incubated at 37°C before and during time-lapse photography. Photographs were captured at a rate of twice per minute for 30 minutes, and are shown in this video at an elapsed rate that is 150 times faster than normal. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2010 |