File:Hypoxia-Transiently-Sequesters-Mps1-and-Polo-to-Collagenase-Sensitive-Filaments-in-Drosophila-pone.0007544.s004.ogv

From Wikimedia Commons, the free media repository
Jump to navigation Jump to search

Original file (Ogg Theora video file, length 1 min 18 s, 1,026 × 1,026 pixels, 162 kbps, file size: 1.5 MB)

Captions

Captions

Add a one-line explanation of what this file represents

Summary

[edit]
Description
English: GFP-Polo localization to mitotic kinetochores and centrosomes increases in response to hypoxia. A syncytial embryo with GFP-Polo and red fluorescently labeled histone (His2AvD-tDimer [37]) completes one cycle of mitosis; a subset of this oocyte is shown in Figure 5B. CO2 gas was turned on after 6:15, and the cell arrested in metaphase of that cell cycle. After 11:30, the gas was turned off, and the embryo resumed mitotic cycling thereafter, reaching metaphase of the next mitotic cycle approximately 25 minutes later. The second cell cycle in this video is prolonged because the onset of hypoxia is much faster than the return to normoxia, as CO2 is quickly pumped into the gas chamber. However, removal of the chamber caused a loss of microscope focus and stage positioning, so to be able to best monitor the oocyte throughout imaging we simply opened the cover to allow ambient air to return by diffusion.
Date
Source Movie S3 from Gilliland W, Vietti D, Schweppe N, Guo F, Johnson T, Hawley R (2009). "Hypoxia Transiently Sequesters Mps1 and Polo to Collagenase-Sensitive Filaments in Drosophila Prometaphase Oocytes". PLOS ONE. DOI:10.1371/journal.pone.0007544. PMID 19847308. PMC: 2761730.
Author Gilliland W, Vietti D, Schweppe N, Guo F, Johnson T, Hawley R
Permission
(Reusing this file)
w:en:Creative Commons
attribution
This file is licensed under the Creative Commons Attribution 2.5 Generic license.
You are free:
  • to share – to copy, distribute and transmit the work
  • to remix – to adapt the work
Under the following conditions:
  • attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
PLOS
PLOS
This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.
Provenance
InfoField
This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
WikiProject Open Access
WikiProject Open Access

File history

Click on a date/time to view the file as it appeared at that time.

Date/TimeThumbnailDimensionsUserComment
current08:13, 27 May 20131 min 18 s, 1,026 × 1,026 (1.5 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

You cannot overwrite this file.

There are no pages that use this file.

Transcode status

Update transcode status
Format Bitrate Download Status Encode time
VP9 720P 28 kbps Completed 06:30, 29 August 2018 9.0 s
VP9 480P 14 kbps Completed 06:30, 29 August 2018 6.0 s
VP9 360P 7 kbps Completed 06:30, 29 August 2018 4.0 s
VP9 240P 4 kbps Completed 06:29, 29 August 2018 3.0 s
WebM 360P 81 kbps Completed 08:14, 27 May 2013 7.0 s
QuickTime 144p (MJPEG) 23 kbps Completed 20:54, 3 November 2024 0.0 s

Metadata

This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.

Retrieved from "https://commons.wikimedia.org/w/index.php?title=File:Hypoxia-Transiently-Sequesters-Mps1-and-Polo-to-Collagenase-Sensitive-Filaments-in-Drosophila-pone.0007544.s004.ogv&oldid=500475291"