File:Hypoxia-Transiently-Sequesters-Mps1-and-Polo-to-Collagenase-Sensitive-Filaments-in-Drosophila-pone.0007544.s003.ogv
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[edit]DescriptionHypoxia-Transiently-Sequesters-Mps1-and-Polo-to-Collagenase-Sensitive-Filaments-in-Drosophila-pone.0007544.s003.ogv |
English: Heat shock does not induce GFP-Polo localization to filaments. A stage 13 GFP-Polo oocyte was heated to 37°C 18 minutes before live imaging commenced. This did not induce GFP localization to filaments. Seven other oocytes on the same slide were also monitored but not live-imaged; all exhibited similar localization before, during and after imaging as the oocyte shown. CO2 was turned on after the first frame, and the filaments appeared shortly thereafter, indicating the cell was still capable of sequestering GFP-Polo to filaments at the elevated temperature. After the eighth time point (5:15), filming was paused while the other seven oocytes were examined. After filming resumed (12:22), the CO2 was turned off, and the GFP-Polo dispersed back into the ooplasm. |
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Source | Movie S2 from Gilliland W, Vietti D, Schweppe N, Guo F, Johnson T, Hawley R (2009). "Hypoxia Transiently Sequesters Mps1 and Polo to Collagenase-Sensitive Filaments in Drosophila Prometaphase Oocytes". PLOS ONE. DOI:10.1371/journal.pone.0007544. PMID 19847308. PMC: 2761730. | ||
Author | Gilliland W, Vietti D, Schweppe N, Guo F, Johnson T, Hawley R | ||
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current | 08:13, 27 May 2013 | 6.5 s, 960 × 540 (431 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Gilliland W, Vietti D, Schweppe N, Guo F, Johnson T, Hawley R |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2009 |