File:Human-T-Lymphotropic-Virus-1-Visualized-at-the-Virological-Synapse-by-Electron-Tomography-pone.0002251.s008.ogv
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Human-T-Lymphotropic-Virus-1-Visualized-at-the-Virological-Synapse-by-Electron-Tomography-pone.0002251.s008.ogv (Ogg Theora video file, length 2.7 s, 337 × 290 pixels, 5.58 Mbps, file size: 1.78 MB)
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[edit]DescriptionHuman-T-Lymphotropic-Virus-1-Visualized-at-the-Virological-Synapse-by-Electron-Tomography-pone.0002251.s008.ogv |
English: Tomographic reconstruction showing a synaptic cleft formed between a chronically HTLV-1-infected MS9 cell (right) and a target Jurkat cell (left). The synaptic cleft is completely enclosed by a close membrane-membrane contact extending over a large area with a diameter of about 6 µm (see Figure 3A and Figure 5A). The membrane-membrane distance in areas of close apposition is about 20 nm. Two HTLV-1 particles are in the synaptic cleft (white arrowheads). White arrows indicate areas in the MS9 cell where viral capsid protein has accumulated below the membrane before directed virus budding. A tomogram slice showing a larger area of the 3D reconstruction can be seen in figure 5A. The sample was fixed with PFA/glutaraldehyde, embedded in Agar 100 resin, post-fixed with OsO4 and stained with lead citrate. The reconstructed 3D volume is composed of tomograms obtained from two serial sections. The tomograms were calculated from tilt series of typically 121 images (tilt range −60° to +60°, 1° degree increment). The images were recorded with a 2k×2k Gatan CCD camera (pixel size 1.54 nm) at an under focus of −0.2 µm with an FEI Tecnai F30 FEG-TEM (300 kV) in Boulder, Colorado using the software SerialEM. The reconstruction was calculated using the software IMOD. The tomogram represents a volume with an area of 1.32×1.92 µm2 and a thickness of 326 nm. The scale bar at the end of the movie corresponds to 500 nm. |
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Source | Movie S8 from Majorovits E, Nejmeddine M, Tanaka Y, Taylor G, Fuller S, Bangham C (2008). "Human T-Lymphotropic Virus-1 Visualized at the Virological Synapse by Electron Tomography". PLOS ONE. DOI:10.1371/journal.pone.0002251. PMID 18509526. PMC: 2386264. | ||
Author | Majorovits E, Nejmeddine M, Tanaka Y, Taylor G, Fuller S, Bangham C | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 10:18, 16 November 2012 | 2.7 s, 337 × 290 (1.78 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Majorovits E, Nejmeddine M, Tanaka Y, Taylor G, Fuller S, Bangham C |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2008 |